The prevalence and antibacterial susceptibility pattern of Enteropathogenic Escherichia coli (EPEC) strains isolated from less than 5 years old children with diarrheal hospitalized in Shahrekord Hajar hospital -2007

Zamanzad, Behnam. and Validi, Majid. and Kheiri, Soleiman. and Maghsoudi, Reza. (2010) The prevalence and antibacterial susceptibility pattern of Enteropathogenic Escherichia coli (EPEC) strains isolated from less than 5 years old children with diarrheal hospitalized in Shahrekord Hajar hospital -2007. journal of shahrekord university of medical sciences, 11 (4).

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Abstract

Background and aim: Enteropathogenic Escherichia coli (EPEC) strains are among the most important diarrheagenic agents in developing countries. This study aimed to determine the common serotypes and antibacterial susceptibility pattern of EPEC strains isolated from less than 5 years old children with diarrhea hospitalized in Shahrekord-Hajar hospital in first six months of 2007. Method: A total of 50 rectal swabs were collected from less than 5 years old children with diarrhea. In addition 50 rectal swabs were obtained from outpatient children without history of diarrhea and gastroenteritis as control group. Stool samples were cultured on differential media EMB and Mac Conkey agar and incubated overnight in 35˚C. Standard biochemical tests (IMVIC) were used for identification of bacteria. Confirmation of isolated bacteria as EPEC strains was performed with specific antisera (Bahar Afshan-Tehran) using slide agglutination method. Besides antibacterial susceptibility pattern of 13 EPEC isolates against some common antibiotics: cephalotin ampicillin nalidixic acid sulfamethoxazol- trimethoprim gentamicin ceftriaxone ciprofloxacine and nitrofurantoin was evaluated using disk diffusion method. Data were analyzed using t-test chi-square and logistic regression. Results: EPEC strains were isolated from 26% of the children with diarrhea (13 patients) compared with 4% of children without diarrhea (2 cases). Our data showed that fifty percent of the EPEC isolates were belonged to O44 O125 O126 and O128 serogroups. In addition 33.3% of the EPEC isolates were belonged to O20 O114 serogroups and finally 16.6% were belonged to O26 O55 and O111 serogroups. Nitrofurantoin ciprofloxacin and gentamicin were the most effective antibiotics against EPEC bacteria. Conclusion: The prevalence of EPEC demonstrates the important role of these strains in causing of acute diarrhea in children. Therefore we suggest the application of routine diagnostic tests for identification and serogrouping of EPEC strains in bacteriologic laboratories.

Item Type: Article
Uncontrolled Keywords: Antibacterial susceptibility Children Acute diarrhea Enteropathogenic Escherchia Coli.
Subjects: WC Communicable Diseases > Infection.Bacterial Infections
QW Microbiology and Immunology
Divisions: Faculty of Health > Department of Epidemiology
Faculty of Medicine > Basic Sciences Academic Groups > Department of Microbiology
Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Reserach Vice-Chancellar Department > Journal of Shahrekord University of Medical Sciences
Reserach Vice-Chancellar Department > Medical Plants Research Center
Depositing User: zahra bagheri .
Date Deposited: 09 Oct 2017 10:27
Last Modified: 10 Mar 2018 04:16
URI: http://eprints.skums.ac.ir/id/eprint/5771

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