Sp1 binding site polymorphism of a collagen gene (rs 1800012) in womenaged 45 and over and its association with bone density

Dehghan, Morteza. and Pourahmad-Jaktaji, Razieh. (2015) Sp1 binding site polymorphism of a collagen gene (rs 1800012) in womenaged 45 and over and its association with bone density. Turkish journal of medical sciences, 45 (3).

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Abstract

Background/aim: Sp1 polymorphism of type I collagen genes is accompanied with bone collagen disorders and severe clinical phenotypes such as osteogenesis imperfecta. The aim of this study was to study the association between COLIA1 Sp1 polymorphism and bone density rate. Materials and methods: In this descriptive, analytical study conducted in 2013 in southwestern Iran, 200 blood samples, per the Cochran sample size formula, were taken from women aged 45 and older. DNA was extracted from the samples using the phenol– chloroform method and the genomic fragments in question were proliferated using the polymerase chain reaction (PCR) method. Results: The genotype distribution of Sp1 polymorphism for the SS, Ss, and ss genotypes was 57.1%, 31.4%, and 11.4%, respectively, in the control group and 9.2%, 75.4%, and 15.4%, respectively, in the patients. Statistically, Sp1 polymorphism in patients had a significant deviation (P = 0.001, χ2 = 34.25) and there was no Hardy–Weinberg equilibrium. In the control group, there was no significant deviation for Sp1 polymorphism (P = 0.226, χ2 = 2.97). Sp1 polymorphism was significantly associated with bone density. Women with the SS genotype had the highest bone density. Conclusion: Sp1 gene polymorphism is associated with bone density rate in women aged 45 and over, and is more commonly observed in homozygosity. Determining this genotype’s polymorphism is valuable to identify the women at risk of developing osteoporosis.

Item Type: Article
Uncontrolled Keywords: Key words: Bone mineral density, collagen type 1α1 Sp1 polymorphism, osteoporosis, polymerase chain reaction
Subjects: WE Musculoskeletal system
WP Gynecology
QU Biochemistry > Cell biology and genetics
Divisions: Faculty of Medicine > Department of Clinical Sciences > Department of Orthopedics
Depositing User: zahra bagheri .
Date Deposited: 25 Sep 2017 04:23
Last Modified: 17 Apr 2018 03:35
URI: http://eprints.skums.ac.ir/id/eprint/5662

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