Homeodomain protein transforming growth factor beta-induced factor 2 like, X-linked function in colon adenocarcinoma cells

Akbari, Abolfazl. and Agah, Shahram. and Heidari, Mansour. and Mobini, Gholam Reza. and Faghihloo, Ebrahim. and Sarveazad, Arash. and Mirzaei, Alireza (2017) Homeodomain protein transforming growth factor beta-induced factor 2 like, X-linked function in colon adenocarcinoma cells. Asian Pacific Journal of Cancer Prevention, 18 (8). pp. 2101-2108.

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Abstract

A member of homeodomain protein namely TGIF2LX has been implicated as a tumor suppressor gene in human malignancy as well as in spermatogenesis. However, to our knowledge, dynamic functional evidence of the TGIF2LX has not yet been provided. The aim of the present study was to investigate the human TGIF2LX target gene(s) using a cDNA-AFLP as a differential display method. A pEGFP-TGIF2LX construct containing the wild-type TGIF2LX cDNA was stably transfected into SW48 cells. UV microscopic analysis and Real-time RT-PCR were used to confirm TGIF2LX expression. The mRNA expressions of TGIF2LX in transfected SW48 cells, the cells containing empty vector (pEGFP-N), and untransfected cells were compared. Also, a Real-time PCR technique was applied to validate cDNA-AFLP results. The results revealed a significant down-regulation and up-regulationby TGIF2LX of Nir1 and Nir2 genes, respectively. The genes are engaged in the cell morphogenesis process. Our findings may provide new insight into the complex molecular pathways underlying colorectal cancer development.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: cDNA-AFLP; TGIF2LX; Transcription factor; Tumor suppressor; Target genes; Colorectal cancer
Subjects: WI Digestive System
QZ pathology-Neoplasms
QU Biochemistry > Cell biology and genetics
Divisions: Faculty of Medicine > Basic Sciences Academic Groups > Department of Biochemistry and Genetics
Depositing User: zahra bagheri .
Date Deposited: 18 Sep 2017 08:10
Last Modified: 21 Feb 2018 05:13
URI: http://eprints.skums.ac.ir/id/eprint/5169

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