Downregulated regulatory T cell function is associated with increased peptic ulcer in Helicobacter pylori-infection

Bagheri, Nader. and shirzad, Hedayatollah. and Elahi, Shokrollah. and Azadegan-Dehkordi, Fatemeh. and Rahimian, Ghorbanali. and Shafigh, Mohammedhadi. and Rashidii, Reza. and Sarafnejad, Abdulfatah. and Rafieian-Kopaei, Mahmoud. and Faridani, Rana. and Tahmasbi, Kamran. and Kheiri, Soleiman. and Razavi, Alireza. (2017) Downregulated regulatory T cell function is associated with increased peptic ulcer in Helicobacter pylori-infection. Microbial Pathogenesis, 110. pp. 165-175.

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Abstract

Background: Helicobacter pylori (H. pylori) chronically colonizes gastric/duodenal mucosa and induces gastroduodenal disease such as gastritis and peptic ulcer and induces vigorous innate and specific immune responses; however, the infection is not removed, a state of chronic active gastritis persists for life if untreated. The objective of this study was to determine the number of regulatory T cells (Tregs) in gastric mucosa of patients with gastritis and peptic ulcer and determined the relationship between main virulence factor of H. pylori and Tregs. Methods and materials: A total of 89 patients with gastritis, 63 patients with peptic ulcer and 40 healthy, H. pylori-negative subjects were enrolled in this study. Expression of CD4 and Foxp3 was determined by immunohistochemistry. Antrum biopsy was obtained for detection of H. pylori, bacterial virulence factors and histopathological assessments. TGF-b1, IL-10 and FOXP3 expressions were determined by real-time polymerase chain reaction (qPCR). Results: The numbers of CD4þ and Foxp3þ T cells as well as the expression of IL-10, TGF-b1, FOXP3, INF-g and IL-17A in infected patients were significantly higher than the ones in uninfected patients. Also, the number of CD4þ T cells was independent on the vacuolating cytotoxin A (vacA) and outer inflammatory protein A (oipA), but it was positively correlated with cytotoxin-associated gene A (cagA). Instead, the number of Foxp3þ T cells was dependent on the vacA and oipA, but it was independent on cagA. The number of Foxp3þ T cells and the expression of IL-10, TGF-b1 and FOXP3 in infected patients with gastritis were significantly higher than the ones in infected patients with peptic ulcer. Moreover, the number of CD4þ T cells and the expression of IL-17A and INF-g was the lowest in the gastritis patients, however, increased progressively in the peptic ulcer patients. Additionally, the numbers of CD4þ and Foxp3þ T cells as well as the expression of IL-10, TGF-b1, FOXP3 and INF-g were positively correlated with the degree of H. pylori density and chronic inflammation. Conclusion: Tregs are positively associated with vacA alleles and oipA status of H. pylori and histological grade but negatively associated with peptic ulcer disease.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: Gastritis; Helicobacter pylori; Peptic ulcer disease; Virulence factor
Subjects: WC Communicable Diseases > Infection.Bacterial Infections
WI Digestive System
QW Microbiology and Immunology
Divisions: Faculty of Health > Department of Epidemiology
Faculty of Medicine > Department of Clinical Sciences > Department of Internal Medicine
Faculty of Medicine > Department of Clinical Sciences > Department of Pathology
Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Reserach Vice-Chancellar Department > Medical Plants Research Center
Depositing User: zahra bagheri .
Date Deposited: 18 Sep 2017 06:21
Last Modified: 30 Jan 2018 08:24
URI: http://eprints.skums.ac.ir/id/eprint/5153

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