In vitro antiviral effects of Peganum harmala seed extract and its total alkaloids against Influenza virus

Moradi, M.-T. and Karimi, A. and Rafieian-Kopaei, M. and Fotouhi, F. (2017) In vitro antiviral effects of Peganum harmala seed extract and its total alkaloids against Influenza virus. Microbial Pathogenesis, 110. pp. 42-49.

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Abstract

This research was aimed to evaluate the in vitro antiviral effect and the mechanism of the effect of Peganum. harmala seeds extract against influenza A virus infection using Madin-Darby canine kidney (MDCK) cells. In this research, ethyl alcohol extract of P. harmala seeds and its total alkaloids was prepared. The potential antiviral activity of the extract and its total alkaloids against influenza A/Puerto Rico/ 8/34 (H1N1; PR8) virus was assessed. The mode of action of the extract to inhibit influenza replication was investigated using virucidal activity, hemagglutination inhibition assay, time of addition assays, RNA replication, western blot analysis and RNA polymerase blocking assay. The crud extract of P. harmala seed and its total alkaloids showed the best inhibitory effect against influenza A virus replication in MDCK cells using MTT assay, TCID50 method and hemagglutination assay. Our results indicated that the extract inhibits viral RNA replication and viral polymerase activity but did not effect on hemagglutination inhibition and virucidal activity. This study showed that, in vitro antiviral activity of P. harmala seed extract against influenza virus is most probably associated with inhibiting viral RNA transcription. Therefore, this extract and its total alkaloid should be further characterized to be developed as anti-influenza A virus agent.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: Influenza A virus Antiviral activity Total alkaloid Peganum harmala L.
Subjects: WC Communicable Diseases > Virus diseases
QV pharmacology > QV 752 Pharmacognosy
Divisions: Reserach Vice-Chancellar Department > Medical Plants Research Center
Reserach Vice-Chancellar Department > student research center
Depositing User: zahra bagheri .
Date Deposited: 18 Sep 2017 06:09
Last Modified: 18 Sep 2017 06:09
URI: http://eprints.skums.ac.ir/id/eprint/5152

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