Investigation of the effects of the essence and extract of Allium jesdianum on the activity of COX-1 and COX-2 enzymes

Khaksarian, Mojtaba. and Gholami, Elham. and Alipour, Maryam. and Sabooteh, Toomaj. and Asadi-Samani, Majid. (2017) Investigation of the effects of the essence and extract of Allium jesdianum on the activity of COX-1 and COX-2 enzymes. INTERNATIONAL JOURNAL OF ADVANCED BIOTECHNOLOGY AND RESEARCH, 8 (2). pp. 1095-1101.

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Abstract

Recent studies on the analgesic effects of Allium jesdianum herb across different types of pain including formalin test biphasic pain, have suggested the probability of effectiveness of the extract of this herb on cyclooxygenase enzymes (COX). In this study we investigated the effects of the essence and extract of A. jesdianum on COX-1 and COX-2 in human fresh blood. For this experimental study, A. jesdianum collected from Sefidkouh region in Khorramabad of Iran. Extract and essence (the leaves of the plant) were prepared at various concentrations 0.5-6 mg/ml and 0-100 mg/ml respectively. The percentage of activity of COX-1 and COX-2 enzyme was measured by percentage of production of TXB2 and PGE2. Solvents Dimethyl sulfoxide (DMSO) and Phosphatebuffered saline (PBS) were used as control for comparing with extract or essence effects. Also indomethacin was used as a positive control. The extract (59 +/- 8.2; P<0.05) and essence (61 +/- 8.7; P<0.05) inhibited COX-1 activity. The extract and essence inhibited COX-2 activity, but it was not significant (P>0.05). Further, all doses of A. jesdianum essence inhibited the platelet aggregation (P<0.05). It can be stated that the essence and extract of A. jesdianum herb had inhibitory effects on COX-1 enzyme, where the extract was also able to inhibit platelet aggregation.

Item Type: Article
Uncontrolled Keywords: Anti-inflammatory agents; Medicinal plants; Pain; Platelet aggregation
Subjects: QU Biochemistry
QV pharmacology > QV 752 Pharmacognosy
Divisions: Reserach Vice-Chancellar Department > student research center
Depositing User: zahra bagheri .
Date Deposited: 18 Sep 2017 04:38
Last Modified: 20 May 2018 05:22
URI: http://eprints.skums.ac.ir/id/eprint/5098

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