THE LACK OF CORRELATION BETWEEN TP53 MUTATIONS AND GASTRIC CANCER: A REPORT FROM A PROVINCE OF IRAN

Saffari-Chaleshtori, Javad. and Tabatabaiefar, Mohammad Amin. and Ghasemi-Dehkordi, Payam. and Farokhi, Effat. and Moradi, Mohammad-Taghi. and Hashemzadeh-Chaleshtori, Morteza. (2017) THE LACK OF CORRELATION BETWEEN TP53 MUTATIONS AND GASTRIC CANCER: A REPORT FROM A PROVINCE OF IRAN. GENETIKA-BELGRADE, 49 (1). pp. 235-246.

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Abstract

Gastric cancer ranks second cause of cancer death worldwide after lung cancer. Its etiology is heterogeneous and genetic factors including protooncogenes and tumor suppressor genes always contribute to the progression of cancer. The TP53 tumor suppressor gene has a broad role in genomic stability and DNA repair. The aim of this study was to determine the TP53 gene mutations in gastric cancer specimens in Chaharmahal Va Bakhtiari province of Iran. In this descriptive-lab based study, we investigated the promoter and exons of TP53 gene mutations in 38 paraffin-embedded gastric cancer specimens. DNA was extracted following a standard phenol-chloroform protocol. The TP53 gene mutations were determined using PCR-SSCP & PCR-RFLP procedures. The present study revealed no TP53 gene mutation in the promoter and exons in the gastric cancer subjects studied. While TP53 gene mutations have been reported as the most frequent genetic alterations and are found in about 50% of the human malignancies, no mutation was detected in this study. This may be due to mutations in other related genes in the same pathway or epigenetic factors.

Item Type: Article
Uncontrolled Keywords: gastric cancer; Iran; PCR-SSCP; RFLP; TP53 gene
Subjects: WI Digestive System
QZ pathology-Neoplasms
Divisions: Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Reserach Vice-Chancellar Department > Clinical Biochemistry Research Center
Depositing User: Users 1 not found.
Date Deposited: 18 Sep 2017 04:11
Last Modified: 16 May 2018 04:54
URI: http://eprints.skums.ac.ir/id/eprint/5094

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