Descriptive molecular epidemiology study of Giardia duodenalis in children of Parana State, Brazil

Matsuchita, H. and Pitz, A. and Melanda, F. and Bregano, R. and Oliveira, F. and Lopes Mori, F. and Menezes, M. and Costa, I. and Pavanelli, W. and Gomes, M. and Colli, C. and Venancio, E. and Conchon-Costa, I. (2017) Descriptive molecular epidemiology study of Giardia duodenalis in children of Parana State, Brazil. International Journal of Epidemiologic Research, 4 (1).

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Abstract

Background and aims: We investigated the children of Parana State, Brazil the prevalence of intestinal parasitosis and the associated factors involved in the transmission of intestinal parasites, and we genotyped the Giardia duodenalis isolates obtained. Methods: Fecal samples were analyzed by established microscopic methods. G. duodenalis positive samples were subjected to genotypic characterization by PCR amplification of sequences of the glutamate dehydrogenase gene (gdh) and by enzymatic digestion with the restriction enzyme NlaIV for classification of genotypes. Results: Of the 877 samples tested, 41% were positive for some intestinal parasitosis, the most common being the presence of protozoa (87.8%). Lack of basic sanitation and poor health education were associated for the intestinal parasite cases found, and the only associated factor for giardiasis was low family income. The G. duodenalis assemblages of gdh amplified samples were 68.6% B and 31.4% AII. Conclusion: These data demonstrate the importance of epidemiological studies for the development of effective strategies with the aim of decreasing the incidence of intestinal parasites in children. Moreover, these results contribute to our knowledge of G. duodenalis assemblages circulating in the world and also offer support for future work on the molecular and clinical aspects of giardiasis.

Item Type: Article
Uncontrolled Keywords: Keywords: Giardiasis, Intestinal parasitosis, Epidemiology.
Subjects: WC Communicable Diseases > Tropical and parasitic Diseases
WI Digestive System
WS Pediatrics
Divisions: Reserach Vice-Chancellar Department > International Journal of Epidemiologic Research
Depositing User: zahra bagheri .
Date Deposited: 12 Sep 2017 06:20
Last Modified: 12 Sep 2017 06:20
URI: http://eprints.skums.ac.ir/id/eprint/4856

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