Cytotoxic effect of essential oil of Artemisia Siberi and Pelargonium roseum on Leishmania major Promastigotes

pirali kheirabadi, Kh. and Hejazi, H. and Layeghi Ghale Soukhteh, S. Cytotoxic effect of essential oil of Artemisia Siberi and Pelargonium roseum on Leishmania major Promastigotes. journal of shahrekord university of medical sciences.

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Abstract

Background and aims: Many studies have performed to find new drugs with activities against Leishmania including chemical and herbal drugs. The aim of this study was to investigate the cytotoxic effects of essential oils of Artemisia Sieberi and Pelargonium roseum against L. major promastigotes compared with Glucantime. Methods: In this experimental study, concentrations of 0.1, 0.2, 0.4, 0.8, 1.2, 1.4, 1.6, and 2 percent of both essences and concentration of 33.8 percent of Glucantime were added to the culture medium included 106 of L. major. The number of viable parasites after adding oils and Glucantime drug at 24, 48 and 72 hours was counted. Each test was repeated four times to ensure repeatability. Data were analyzed using SAS software and Tukey test. Results: The number of viable parasites after adding the herbal essences significantly decreased in comparison with culture medium included Glucantime (P&le0.05). 72 hours after exposure with glucantime and herbal essences to parasite, the number of parasites decreased from 1.3×106 parasite per ml in control group to 6.06× 105 parasite per ml in treated group with glucantime and 1.6×105 and 1.7×105 in groups treated with essences of Pelargonium and Artemisia, respectively. Conclusion: Because of phylogenetic similarity between promastigot and amastigot forms of leishmania, effect of Artemisia sieberi and Pelargonium roseum oils on the amastigot of parasite is possible and use of such oils for treatment and control of amastigot form of parasite is suggested. More study is needed to verify and use this biomedical agent as an alternative potential instead of chemicals. The results of this study showed that despite the increasing resistance of promastigotes of Leishmania to chemical drugs, essential oils of plant origin can be used as an alternative therapy for the treatment of CL and the essential oils have potential effect to inhibit the proliferation of Leishmania parasites.

Item Type: Article
Uncontrolled Keywords: Artemisia siberi, Pelargonium roseum, Leishmania major, Glucantime.
Subjects: QV pharmacology > QV 752 Pharmacognosy
QW Microbiology and Immunology
QX Parasitology
Divisions: Reserach Vice-Chancellar Department > Journal of Shahrekord University of Medical Sciences
Depositing User: zahra bagheri .
Date Deposited: 11 Sep 2017 08:03
Last Modified: 11 Sep 2017 08:04
URI: http://eprints.skums.ac.ir/id/eprint/4827

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