Effect of Phoenix dactylifera pollen grain on maturation of preantral follicles in NMRI mice

Salek Abdollahi, Farzaneh. and Baharara, Javad. and Nejad Shahrokhabadi, Khadijeh. and Namvar, Farideh. and Amini, Elaheh. (2015) Effect of Phoenix dactylifera pollen grain on maturation of preantral follicles in NMRI mice. Journal of Herbmed Pharmacology, 4.

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Abstract

<p><strong>Introduction:</strong> Optimizing an ideal culture system that is comparable to follicle environment to obtain mature oocytes is one of the important criteria in in vitro maturation (IVM). Phoenix dactylifera L. commonly known as date palm is an important herb in Asia folk medicine that is used to improve fertility in women from ancient time. The aim of this study was to investigate the effect of date palm pollen grain extract on IVM of mouse follicles. </p> <p><strong>Methods:</strong> In this study follicles with 1 or 2 layers of granulosa cells and round oocytes were isolated from 2-3 weeks old female NMRI mice ovaries. Follicles were cultured in IVM media with different concentrations 0, 10, 20, 30, 40 &mu;g/ml of palm pollen grain extract for 12 days. Then, the effect of date palm pollen grain on follicular growth and maturation were analyzed. </p> <p> <strong>Results: </strong>There was a significant increase in follicle growth and maturation rate in all treated groups as compared to the control group, but maturation rate was significantly higher in the presence of 20 &mu;g/ml palm pollen grain. </p> <p><strong>Conclusion:</strong> Supplementation of IVM media with date palm pollen grain extract improves the IVM of follicles.</p>

Item Type: Article
Uncontrolled Keywords: Follicle,Oocyte,In vitro maturation (IVM),Date palm pollen grain
Subjects: QV pharmacology > QV 752 Pharmacognosy
Divisions: Reserach Vice-Chancellar Department > Journal of Herbmed Pharmacology
Depositing User: zahra bagheri .
Date Deposited: 10 Sep 2017 07:05
Last Modified: 29 May 2018 05:44
URI: http://eprints.skums.ac.ir/id/eprint/4777

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