Effect of Crocus sativus Stigma (saffron) alone or in combination with chloroquine on chloroquine sensitive strain of Plasmodium berghei in mice

Pestechian, Nader. and Abedi-Madiseh, Somayeh. and Ghanadian, Mustafa. and Nateghpour, Mehdi. (2015) Effect of Crocus sativus Stigma (saffron) alone or in combination with chloroquine on chloroquine sensitive strain of Plasmodium berghei in mice. Journal of Herbmed Pharmacology, 4.

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Abstract

Introduction: In malaria treatment protocols, treatment failure or drug resistance of synthesized drugs like alkaloids related to quinine, and aminoquinolines are the main problems now. Therefore, discovering efficient drugs or combination therapy of blood schizonticidal drugs with different mechanisms or different targets in the parasite is a crucial effort to solve this problem. In this study, the effectiveness of Crocus sativus Stigma (saffron) individually and in combination with chloroquine, was considered against chloroquine–sensitive strain of Plasmodium berghei.Methods: At the first stage, using 4 day suppressive Peter’s test in mice, ED50 and survival times of saffron methanol extract, and its aqueous and ethyl acetate fractions and chloroquine on P. berghei were calculated. Then, based on the toxicity and survival time results, combination therapy was conducted with the best saffron fraction and chloroquine against the parasite.Results: The saffron extract, aqueous and ethyl acetate fractions resulted in suppression of parasitemia with ED50 values of 587.0 ± 78.7, 323.7 ± 37.2, and 508.7 ± 35.6 mg/kg, respectively. Combination of ethyl acetate fraction with chloroquine, potentiated the antimalarial property and the survived percent of the treated mice on days 7, 14, and 28 significantly more than chloroquine or ethyl acetate fraction alone.Conclusion: Saffron and its fractions individually can be effective in reducing the parasitemia in mice. The outcome of combination of ethyl acetate fraction with chloroquine on the mice showed synergistic effect on the chloroquine–sensitive strain of parasite.

Item Type: Article
Uncontrolled Keywords: Crocus sativus Saffron Plasmodium berghei Combination therapy Chlor
Subjects: QV pharmacology > QV 752 Pharmacognosy
Divisions: Reserach Vice-Chancellar Department > Journal of Herbmed Pharmacology
Depositing User: zahra bagheri .
Date Deposited: 10 Sep 2017 10:32
Last Modified: 15 Apr 2018 07:52
URI: http://eprints.skums.ac.ir/id/eprint/4764

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