In vitro evaluation of antiviral activity of essential oil from <em>Zataria multiflora</em> Boiss. against Newcastle disease virus

Mohammadian-Hafshejani, Ali and Mosleh, Najmeh. and Shomali, Tahoora. and Ahmadi, Maryam. and Sabetghadam, Sara. (2015) In vitro evaluation of antiviral activity of essential oil from <em>Zataria multiflora</em> Boiss. against Newcastle disease virus. Journal of Herbmed Pharmacology, 4.

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Abstract

<p><strong>Introduction: </strong>The study aimed to investigate the antiviral activity of Zataria multiflora (ZM) essential oil against Newcastle disease virus (NDV) on Vero cells. </p> <p><strong>Methods: </strong>The cytotoxicity of ZM essential oil was evaluated by MTT assay. Cells were infected with 100 TCID50 of a field isolate of virulent NDV (JF820294.1). ZM essential oil at concentrations of 1/5000, 1/25 000, 1/125 000 or 1/625 000 was added at different times of infection: 60 minutes pre infection, simultaneously and 60 minutes post infection. Cells were evaluated morphologically. The TCID50, neutralizing index (NI) and HA titer were determined. </p> <p> <strong>Results:</strong> Cells treated with ZM essential oil in all concentrations 1 hour before or after infection, showed CPE similar to control virus cells. In simultaneous use, cells treated with 1/5000 concentration of the essential oil, remained morphologically normal. TCID50 values of all treatments were very close to that of control virus except for simultaneous administration at concentration of 1/5000 which was about 1000 folds lower. Virus titer in different treatments was exactly the same as control virus titer in Haemagglutination (HA) test. </p> <p> <strong>Conclusion: </strong>ZM essential oil has some antiviral activity on NDV in vitro, which is possibly by destruction of virus infectivity or inhibition of early phases of viral proliferation cycle.</p>

Item Type: Article
Uncontrolled Keywords: Newcastle disease virus Zataria multiflora Vero cells Anti viral activity
Subjects: WC Communicable Diseases > Virus diseases
QV pharmacology > QV 752 Pharmacognosy
Divisions: Reserach Vice-Chancellar Department > Journal of Herbmed Pharmacology
Depositing User: zahra bagheri .
Date Deposited: 10 Sep 2017 09:35
Last Modified: 20 May 2018 05:14
URI: http://eprints.skums.ac.ir/id/eprint/4755

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