Ghassemi-Dehkordi, Nasrollah. and Ghanadian, Mostafa. and Arabha, Sajjad. (2014) Development of a validated HPLC method for the determination of sennoside A and B, two major constituents of Cassia obovata Coll. Journal of HerbMed Pharmacology, 3.
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Abstract
<p> <strong>Introduction: </strong><em>Cassia obovata</em> Coll is the only <em>Senna </em>species which grows wild in Iran. In the present study, an optimised reverse High Performance Liquid Chromatography (HPLC) validated method was established for quantification of sennosides A and B, the major constituents of <em>C. obovata</em> with a simple and accurate method. </p> <p><strong>Methods:</strong> HPLC analysis was done using Waters 515 pump on a Nova-Pak C18 (3.9 × 150 mm). Millennium software was used for the determination of the sennoside A and B in Cassia species and processing the information. The method was validated according to USP 32 requirements.</p> <p> <strong>Results:</strong> The solvent impact on the selectivity factor and partition coefficient parameters evaluated. Using a conventional RP-18 L1 column, 3.9 × 150 mm, the mobile phase was selected after several trials with different mixtures of water and acetonitrile. Sennosides A and B were determined using the external standard calibration method. Using USP 35-NF 30, the LOD and LOQ were calculated. The reliability of the HPLC-method for analysis of sennoside A + B was validated through its linearity, reproducibility, repeatability, and recovery. Fina1ly ethanol:water (1:1) extracts of <em>Cassia obovata</em> and <em>Cassia angustifolia </em>were standardized by assay of sennoside A and B through above HPLC validated method. </p> <p><strong>Conclusion:</strong> Through the above method, determination of sennosides in Cassia species are completely possible. Moreover, through comparing the results, even though sennosides are rich in <em>Cassia angustifolia</em> but, the results shows that <em>C. obovata</em> could be considered as an alternative source for sennosides A and B. </p>
Item Type: | Article |
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Uncontrolled Keywords: | Cassia obovata Sennoside A Sennoside B High Pressure Liquid Chromatography |
Subjects: | QV pharmacology > QV 752 Pharmacognosy |
Divisions: | Reserach Vice-Chancellar Department > Journal of Herbmed Pharmacology |
Depositing User: | Users 1 not found. |
Date Deposited: | 10 Sep 2017 07:41 |
Last Modified: | 03 Feb 2018 08:44 |
URI: | http://eprints.skums.ac.ir/id/eprint/4739 |
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