Anti-angiogenesis effect of <em>crocous sativus </em>L. extract on matrix metalloproteinase gene activities in human breast carcinoma cells

Mousavi, Marzieh. and Baharara, Javad. and Asadi-Samani, Majid. (2014) Anti-angiogenesis effect of <em>crocous sativus </em>L. extract on matrix metalloproteinase gene activities in human breast carcinoma cells. Journal of HerbMed Pharmacology, 3.

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Abstract

<p><strong>Introduction: </strong>There is an interest in <em>Crocous sativus L</em>. (Saffron) mainly because of its biological properties. Biomedical research has focused on saffron as a powerful antioxidant, anti-inflammatory and anti-tumor, but its mechanism has not yet been thoroughly clarified. In this study, the effects of saffron aqua extract on matrix metalloproteinases (MMP) gene expression were investigated. </p> <p><strong>Methods:</strong> In this experimental study, the saffron was extracted using water as solvent. MCF-7 cells in RPMI1640 medium were supplemented with 10 FBS and incubated at 37 ˚C with 5 CO2. After 24 h, the cells were treated by saffron extract at concentrations of 100, 200, 400 and 800 &mu;g/ml. 48 h after treatment, total RNA was extracted and cDNA was synthesized using specific primer. Synthesized products were analyzed by Real Time PCR to determine expression level of MMP. </p> <p> <strong>Results:</strong> Data analysis showed inhibitory effect of saffron at concentrations of 100 to 800 &mu;g/ml on MMP gene expression in comparison with control group. Reduction for 100, 200, 400 and 800 &mu;g/ml were 5, 18, 15, 11, respectively. According to data analysis treating MCF-7 cells with saffron at concentration of 200 &mu;g/ml caused the highest decrease, with 18 reduction in gene expression (P&lt;0.001).</p> <p> <strong>Conclusion:</strong> Results indicate decrease in the expression of MMP compared with controls revealing induction of inhibitory effects of saffron on angiogenesis which might be also considered as a promising chemotherapeutic agent in breast cancer treatment.</p>

Item Type: Article
Uncontrolled Keywords: Crocous Sativus L Angiogenesis Matrix metalloproteinase MCF-7 Cell Line Cancer
Subjects: QZ pathology-Neoplasms
QU Biochemistry > Cell biology and genetics
Divisions: Reserach Vice-Chancellar Department > Journal of Herbmed Pharmacology
Depositing User: zahra bagheri .
Date Deposited: 10 Sep 2017 07:31
Last Modified: 04 Feb 2018 08:17
URI: http://eprints.skums.ac.ir/id/eprint/4737

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