Development of a validated HPLC method for determination of an active component in Pycnocycla spinosa and tablets prepared from its extract

Tavakolli, Naser. and Ghanadian, Mustafa. and Asghari, Gholamreza. and Sadraei, Hassan. and Asgari Borjlou, Nadia. and Tabakhian, Majid. (2017) Development of a validated HPLC method for determination of an active component in Pycnocycla spinosa and tablets prepared from its extract. Journal of Herbmed Pharmacology, 6.

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Abstract

Introduction: Pycnocycla spinosa, a native plant of Iran with approved antispasmodic and antidiarrheal activities, could be a suitable candidate and an alternative remedy for the treatment of diarrhea and irritable bowel syndrome (IBS). Therefore, the aim of this study is formulation of an acceptable dosage form and development of a validated high-performance liquid chromatography (HPLC) method for analysis of active ingredients in its extract and pharmaceutical forms.Methods: Different formulations of P. spinosa tablets were prepared by wet granulation method. The prepared tablets were evaluated for hardness, friability, disintegration time and drug assay. HPLC was carried out based on the extract active ingredient: 6-(4-hydroxy-3-methoxyphenyl)-hexanoic acid (HMPHA) determination in P. spinosa extract and tablets.Results: The mean weight, friability, hardness, and disintegration time of selected formulation (tablet 5 mg) were 217.26 mg, 0.69, 53.6 N and 95.8 seconds, respectively. Similar acceptable results were also found for 10 mg tablets. The assay test showed that the content of HMPHA in each 5 mg and 10 mg tablets were 1.64 μg and 3.59 μg, respectively. The HPLC method showed a good linearity and suitability in its working range: 4.5 to 15 μg/mL.Conclusion: The data showed that the selected formulation of P. spinosa tablets has acceptable physicochemical features.

Item Type: Article
Uncontrolled Keywords: Umbelliferae Pycnocycla spinosa Formulation Apigenin Diarrhea IBS HPLC
Subjects: WI Digestive System
QV pharmacology > QV 752 Pharmacognosy
Divisions: Reserach Vice-Chancellar Department > Journal of Herbmed Pharmacology
Depositing User: zahra bagheri .
Date Deposited: 10 Sep 2017 04:55
Last Modified: 25 Mar 2018 04:39
URI: http://eprints.skums.ac.ir/id/eprint/4714

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