Method validation of methotrexate in human plasma by LC-MS technique in patients with brain tumor.

Danafar, H. (2016) Method validation of methotrexate in human plasma by LC-MS technique in patients with brain tumor. International Journal of Epidemiologic Research, 3 (2). pp. 162-171.

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Abstract

Background and aims: A selective and sensitive high performance liquid chromatography-electrospray ionization mass spectrometry method has been established for determination of methotrexate in human plasma. Methods: Methotrexate was extracted from plasma with acetonitrile. The mobile phase consisted of acetonitrile-water-formic acid 74: 25: 1(v/v). Twenty µl of sample was chromatographically analyzed using a repacked ZORBAX-XDB-ODS C18 column (2.1mm×30mm, 3.5 micron). The mode of mass spectrometry was selected-ion monitoring (SIM). Results: Standard curve was linear (r= 0.998) over the concentration range of 0.1–100.0 ng/ml and showed a suitable accuracy and precision. The limit of detection (LOD) was 0.05ng/ml. Conclusion: The method is quick, easy, very steady and precise for the partition, assignment and evaluation of methotrexate in human plasma.

Item Type: Article
Uncontrolled Keywords: Methotrexate; LC-MS; Human plasma
Subjects: WL Nervous system
QV pharmacology > QV 704 Pharmaceutics
Divisions: Reserach Vice-Chancellar Department > International Journal of Epidemiologic Research
Depositing User: zahra bagheri .
Date Deposited: 05 Sep 2017 08:36
Last Modified: 05 Sep 2017 08:36
URI: http://eprints.skums.ac.ir/id/eprint/4539

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