Monoclonal antibodies production against a 40KDa band of hydatid cyst fluid.

Yousofi Darani, Hossein. and Ataei, Behrooz. and Sharafi, Seyedeh Maryam. and shirzad, Hedayatollah. and KhanAhmad, Hossein. (2017) Monoclonal antibodies production against a 40KDa band of hydatid cyst fluid. Recent patents on biotechnology. ISSN 2212-4012

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Abstract

BACKGROUND AND OBJECTIVES Hydatid cyst is the larval stage of the tapeworm Echinococcus granulosus. Hydatid cyst fluid, cyst membrane and Protoscolices, contain a complex mixture of antigens that can induce immune responses in the host. Anti-cancer properties of Protoscolices and hydatid cyst fluid has been shown. In order to identify antigens of hydatid cyst fluid that have anti-cancer effect, in this study production of monoclonal antibodies against one of the hydatid cyst fluid band (40KDa) has been investigated. There are many published patents about applications of monoclonal antibodies. METHOD In this experimental study, 40KDa band of hydatid cyst fluid that has cross reaction with sera of patients with breast cancer was used as antigen. A group of mice were immunized with this antigen, and then their spleen cells were extracted and fused with SP2 cells. Monoclonal antibodies production was checked in wells with signs of cell growth using ELISA and western blotting. The reaction of the produced monoclonal antibodies with breast cancer cells was tested using flow cytometry method. Finally effect of the monoclonal antibodies on growth of breast cancer cells was investigated in vitro. RESULT The results of this study showed that in the first plate antibody against 40KDa was detected in several wells. In the second plate monoclonal antibodies with high titer was detected in one well. The produced monoclonal antibodies reacted with the surface of breast cancer cells. However, they had no significant effect on growth of breast cancer cells in culture medium. CONCLUSION Monoclonal antibodies against hydatid cyst fluid 40KDa band were produced. These antibodies reacted with the surface of breast cancer cells but had no significant effect on growth of these cells.

Item Type: Article
Uncontrolled Keywords: anti-tumor ; hydatid cyst antigens; monoclonal antibodies
Subjects: WH Hemic and Lymphatic System
Divisions: Faculty of Medicine > Basic Sciences Academic Groups > Department of Immunology
Depositing User: Users 1 not found.
Date Deposited: 25 Jun 2017 09:31
Last Modified: 04 Apr 2018 04:24
URI: http://eprints.skums.ac.ir/id/eprint/370

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