Epidemiology of Rotavirus Diarrhoea in Iranian Children

Cuevas, L. and Khalili, B. and Reisi, N. and Dove, W. and Cunliffe, N. and Hart, C. (2004) Epidemiology of Rotavirus Diarrhoea in Iranian Children. Journal of Medical Virology, 73 (2). pp. 309-312.


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Human rotavirus is the most important cause of severe diarrhoea in infants and young children worldwide. We describe the aetiology of viral diarrhoea and the characteristics of rotavirus infection in Shahrekord, Iran. Two hundred and fifty nine children <5 years old admitted to Hajar Hospital, 245 children with acute diarrhoea attending primary health centres in Shahrekord, and 114 children hospitalised for elective surgery were selected from October 2001 to August 2002. Stool samples were screened for enteric viruses using EM. Rotaviruses were characterised using ELISA, reverse transcription-polymerase chain reaction (RT-PCR), and electropherotyping. One hundred and eighty six viruses were identified, of which 146 (78) were rotavirus. The second most frequent virus was coronavirus, followed by calicivirus. Rotaviruses exhibited a marked seasonal variation, being most frequently isolated from November to February (50 of rotavirus recovered) and affected mostly children <2 years old. The RT-PCR successfully typed 139 of the 146 (95) rotavirus G types and 124 (85) P types. The most frequent P type was, P8 in 108 (74%), P4 in 16 (11%), and was P non-typeable in 22 (15%). Among the G types, G1 was identified in 120 (82%), G2 in 19 (13%), and was G-non-typeable in 7 (5%). Our results are the first report of rotavirus genotypes affecting Iranian children. The most frequent G and P types (G1, G2, P8, and P4) are similar to those reported from around the world and will be covered by existing rotavirus vaccines targeting G types G1-G4. © 2004 Wiley-Liss, Inc.

Item Type: Article
Additional Information: cited By
Uncontrolled Keywords: Rotavirus vaccine, article; Calicivirus; childhood disease; controlled study; Coronavirus; diarrhea; disease severity; elective surgery; electropherotyping; enzyme linked immunosorbent assay; feces analysis; female; genotype; human; infant; Iran; major clinical study; male; preschool child; reverse transcription polymerase chain reaction; Rotavirus; rotavirus g; rotavirus g1; rotavirus g2; rotavirus g3; rotavirus g4; virus infection, Antigens, Viral; Caliciviridae; Child, Preschool; Coronavirus; Diarrhea; Electrophoresis, Agar Gel; Enzyme-Linked Immunosorbent Assay; Feces; Female; Genotype; Humans; Infant; Iran; Male; Microscopy, Electron; Reverse Transcriptase Polymerase Chain Reaction; Rotavirus; Rotavirus Infections; Seasons, Caliciviridae; Coronavirus; Human rotavirus A; Irania; Rotavirus
Subjects: WC Communicable Diseases > Virus diseases
WS Pediatrics
Divisions: Faculty of Medicine
Depositing User: Users 1 not found.
Date Deposited: 21 Aug 2017 09:55
Last Modified: 21 Aug 2017 09:55
URI: http://eprints.skums.ac.ir/id/eprint/3470

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