Molecular detection of prostate specific antigen in patients with prostate cancer or benign prostate hyperplasia the first investigation from Iran

Andonian, Laris. and Khorramizadeh, M.R. and Farhud, Daryoush. and Hashemzadeh-Chaleshtori, Morteza. and Holakouie Naieni, K. and Razi, A. and Sanadizadeh, J. and Pourmand, G. and Nouraie, M. and Rezaie, S. and Saadat, F. and Yepiskoposyan, L. and Norouzi, M. and Soleimanpour, H. and Berahme, A. and Aalizadeh, N. (2005) Molecular detection of prostate specific antigen in patients with prostate cancer or benign prostate hyperplasia the first investigation from Iran. Iranian Journal of Public Health, 34 (3). pp. 20-26.


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Prostate cancer is the second common form of cancer in men. Detection of circulating Prostate Specific Antigen (PSA) transcripts has effectively been used for early diagnosis of prostate cancer cells. This investigation employed a reverse transcriptase polymerase chain reaction (RT-PCR) technique to distinguish the patients with either localized or metastatic prostate cancer (CaP) vs. Benign Prostate Hyperplasia (BPH) and control subjects, as compared with clinical and pathological records. With reservation of ethical issues, blood samples were collected from 60 cases. Based on pathological and clinical findings, 25 patients (20 with localized cancer, 5 with metastatic), 22 with BPH, and 13 healthy (including 3 females) subjects as negative controls, were selected from Shariati, Mehrad, Sina,, Khatam and Atie Hospitals in Tehran, Iran. RT-PCR for a 260 bp PSA transcript was then performed. Clinical and pathological records were used for the assessment and comparison of PSA RT-PCR results. None of the control subjects and BPH (with 7 exceptions) were found positive by RT-PCR (Relative specificity= 72.7). In patients with prostate cancer, 21 out of 25 were found PSA positive (Relative sensitivity= 83.4) and the remaining 3 have been shown to be PSA negative (Positive predictive value= 83.4). All of 5 metastatic patients (100) revealed PSA positive results. Our data reflects the clinical relevance and significance of RT-PCR results as assessed with clinical and pathological examinations. PSA RT-PCR might be used as a powerful means for diagnosis, even when either pathological or clinical findings are negative, and could be employed for further molecular epidemiology surveys.

Item Type: Article
Additional Information: cited By
Uncontrolled Keywords: complementary DNA; prostate specific antigen; RNA, adult; aged; antigen detection; article; blood sampling; cancer screening; controlled study; correlation analysis; diagnostic accuracy; diagnostic value; female; human; human cell; Iran; major clinical study; male; metastasis potential; prediction; prostate cancer; prostate hypertrophy; reverse transcription polymerase chain reaction; RNA analysis; sensitivity and specificity; statistical significance
Subjects: WJ Urogenital System
QZ pathology-Neoplasms
QU Biochemistry > Cell biology and genetics
Divisions: Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Depositing User: Users 1 not found.
Date Deposited: 21 Aug 2017 08:44
Last Modified: 28 May 2018 04:37

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