Expression and in vitro characterization of herpes simplex virus 1 (HSV-1) ORF P protein

Karimi, A. and Nafisi, M.R. (2006) Expression and in vitro characterization of herpes simplex virus 1 (HSV-1) ORF P protein. Iranian Biomedical Journal, 10 (1). pp. 9-13.

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Abstract

Herpes simplex virus 1 (HSV-1) unspliced 8.3 latency associated transcript (LAT), which located in the long repeat sequences, has been shown to contain at least 16 open reading frames (ORF: A-P). One of these ORF, ORF P, maps almost entirely antisense to HSV-1 neurovirulence gene, ICP34.5. Both ORF P and ICP34.5 are located in the long repeat and are antisense overlapping genes. Therefore, in ORF P deletion mutants, ICP34.5 is also deleted and thus, the characterization of ORF P mutants is almost impossible. An alternative way to analyse its function is to determine those cellular and viral proteins which interact with ORF P. During these experiments, firstly, the expression of full length Glutatione-S-transferase (GST)-ORF P fusion protein was optimised and then, using GST-pull down, it was shown that ORF P interacts with a viral and a few cellular proteins in vitro. Conclusively, ORF P might have some functions in HSV-1 replication cycle.

Item Type: Article
Additional Information: cited By
Uncontrolled Keywords: glutathione transferase; glycine cleavage system; hybrid protein; virus protein, article; controlled study; deletion mutant; gene function; gene location; gene mapping; Herpes simplex virus 1; in vitro study; long terminal repeat; nonhuman; nucleotide repeat; open reading frame; protein expression; protein function; protein protein interaction; virus latency; virus replication; virus virulence, Human herpesvirus 1
Subjects: WC Communicable Diseases > Virus diseases
QU Biochemistry
Divisions: Faculty of Medicine > Basic Sciences Academic Groups > Department of Immunology
Faculty of Medicine > Basic Sciences Academic Groups > Department of Microbiology
Depositing User: zahra bagheri .
Date Deposited: 21 Aug 2017 08:37
Last Modified: 21 Aug 2017 08:37
URI: http://eprints.skums.ac.ir/id/eprint/3439

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