Detection and molecular characterization of Cryptosporidium Species in recreational waters of Chaharmahal va Bakhtiyari Province of Iran using nested PCR-RFLP

Manouchehri Naeini, K. and Asadi, M. and Hashemzade Chaleshtori, M. (2011) Detection and molecular characterization of Cryptosporidium Species in recreational waters of Chaharmahal va Bakhtiyari Province of Iran using nested PCR-RFLP. Iranian Journal of Parasitology, 6 (1). pp. 20-27.

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Abstract

Background: The aim of this study was to detect and characterize Cryptosporidium spp. in water samples collected from recreational ponds of Chaharmahal va Bakhtiyari Province of Iran. Methods: Thirty water samples were collected from November 2009 to May 2010. Each sample contained 10 liters of water. We used the SSU rRNA-based PCR-RFLP technique. Results: Out of thirty samples examined, 6 (20) were positive for different Cryptosporidium spp. Restriction pattern analysis showed that C. parvum has been the most prevalent genotype, followed by C. hominis and C. canis, respectively. In this area, the higher prevalence of C. parvum compared with other genotypes is consistent with the distribution of cattle. Conclusion: Farm animals, particularly cattle are the main source of cryptosporidial contamination for recreational waters in this area.

Item Type: Article
Additional Information: cited By
Uncontrolled Keywords: ribosome RNA, article; bacterium detection; cattle; Cryptosporidium; Cryptosporidium canis; Cryptosporidium hominis; genotype; Iran; nonhuman; polymerase chain reaction; prevalence; restriction fragment length polymorphism; water contamination; water sampling, Animalia; Bos; Cryptosporidium; Cryptosporidium canis; Cryptosporidium hominis; Cryptosporidium parvum
Subjects: WA Public Health > Public Health Education WA.18 > WA 30 Social, Economic, and Environmental factor in public health
WA Public Health > WA 30 Social, Economic, and Environmental factor in public health
Divisions: Faculty of Medicine > Basic Sciences Academic Groups > Department of Parasitology and Mycology
Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Depositing User: zahra bagheri .
Date Deposited: 20 Aug 2017 07:59
Last Modified: 20 Aug 2017 07:59
URI: http://eprints.skums.ac.ir/id/eprint/3296

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