Effect of Euphorbia helioscopia extract on acute and chronic pain in mice

Shirani, Manoochehr. and Alibabaei, Zahra. and Kheiri, Soleiman. and shirzad, Hedayatollah. and Taji, Fatemeh. and Asgari, Azam. and Rafieian-Kopaei, Mahmoud. (2011) Effect of Euphorbia helioscopia extract on acute and chronic pain in mice. Journal of Babol University of Medical Sciences, 13 (4).


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Most of analgesics have side effects and introducing new drags is essential. Euphorbia helioscopia is a medicinal plant which is used by people in Chaharmahal Bakhtiary province of Iran as a painkiller. This experiment was performed to study the analgesic effect of an alcoholic extract from the aerial parts of Euphorbia helioscopia by formalin test. METHODS: In an experimental study 100 Balb/c mice were divided into 10 groups as follows: group 1 received distilled water, group 2 to 7 received 0.05, 0.1, 0.2, 0.4, 2 and 8mg/mouse of alcoholic extract, respectively. Group 8 received 6 mg/kg ibuprofen, group 9, 1 mg/kg naloxone and group 10 received naloxone and extract. The pain-related behavior was counted for a period of 30 min, following injection of 20 microliter of 1.5 formalin. The data obtained in case groups were compared with the ones in control group. The plant was extracted by maceration method using ethanol 96. The extract was then concentrated and reconstructed in normal saline to produce different desired concentrations. FINDINGS: In the first 5 minutes (acute period) of the experiment 8mg/mouse of the extract and in the following 25 minutes, 0.1 and 8mg/mouse of the extract reduced pain reaction, compared to control group (p<0.05). Naloxone had no effect on this effect of the extract. CONCLUSION: Euphorbia helioscopia has antinociceptive activity that is not due to interaction with opioid system.

Item Type: Article
Additional Information: cited By
Uncontrolled Keywords: alcohol; Euphorbia extract; Euphorbia helioscopia extract; formaldehyde; ibuprofen; naloxone; unclassified drug, acute disease; analgesic activity; animal experiment; animal model; antinociception; article; chronic pain; controlled study; drug isolation; Euphorbia; Euphorbia helioscopia; Iran; medicinal plant; mouse; nonhuman; pain
Subjects: QV pharmacology > QV 752 Pharmacognosy
Divisions: Reserach Vice-Chancellar Department > Medical Plants Research Center
Depositing User: Users 1 not found.
Date Deposited: 20 Aug 2017 06:43
Last Modified: 15 May 2018 06:32
URI: http://eprints.skums.ac.ir/id/eprint/3280

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