Development of a Latex Agglutination Test as a Simple and Rapid Method for Diagnosis of Trichomonas vaginalis Infection.

Yousofi Darani, H. and Ahmadinejad, F. and Zabardast, N. and Yousefi, H.A. and Shirzad, H. (2010) Development of a Latex Agglutination Test as a Simple and Rapid Method for Diagnosis of Trichomonas vaginalis Infection. Avicenna journal of medical biotechnology, 2 (1). pp. 63-6. ISSN 2008-2835

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Abstract

Trichomoniasis is a worldwide infection and due to its complications rapid and accurate diagnosis of infection especially in pregnant women is very important. In this study, development of a latex agglutination test using native antigens for rapid diagnosis of trichomoniasis is investigated. Trichomonas vaginalis was harvested from TYIS33 culture medium and anti Trichomonas vaginalis antiserum was raised in rabbits. Salt precipitation method was used for antibody purification. Polyesteren latex particles coated with purified antibody and used for detection of Trichomonas vaginalis. Clinical samples of vaginal discharge were collected from 500 women and examined for Trichomonas vaginalis by using wet mount, culture and latex agglutination tests. Sensitivity and specificity of latex test was determined considering culture as golden standard. Sensitivity and specificity of latex agglutination test was 100% and 81% and those of wet mount were 33.3% and 100%, respectively. Positive and negative predictive values of latex agglutination test were 6% and 100%, respectively. Due to inconvenient sensitivity and specificity of the latex agglutination test developed in this study, further work is recommended to improve the test.

Item Type: Article
Uncontrolled Keywords: Latex agglutination test, Sensitivity, Specificity, Trichomonas vaginalis
Subjects: WC Communicable Diseases > Infection.Bacterial Infections
WJ Urogenital System
Divisions: Faculty of Medicine > Basic Sciences Academic Groups > Department of Immunology
Faculty of Medicine > Basic Sciences Academic Groups > Department of Parasitology and Mycology
Depositing User: zahra bagheri .
Date Deposited: 20 Aug 2017 04:35
Last Modified: 20 Aug 2017 04:35
URI: http://eprints.skums.ac.ir/id/eprint/3212

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