Expression of NC-2 Receptor on MCL Cells and Its Natural Cytotoxicity Against Cancer Cells

Shirzad, H. and Zamanzad, B. and Shahabi, Gh.A. (2009) Expression of NC-2 Receptor on MCL Cells and Its Natural Cytotoxicity Against Cancer Cells. KUWAIT MEDICAL JOURNAL, 41 (2). pp. 112-116.

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Abstract

Objectives: To identify the expression of NC-2 on an interleukin-3 dependent mast cell line (MCL) and investigate the activity of this receptor against tumor cells Design: Laboratory study Setting: Cellular and Molecular Center, Shahrekord, Iran Subjects and Methods: The MCL cells were stained with D9 monoclonal antibody (anti-NC-2) and analysed by flow cytometry. This was confirmed by immunoperoxidase staining. The cytotoxicity assay was performed to show the cytotoxic activity of MCL cells against 51Cr-labelled WEHI-164 tumor cells. Intervention: The expression of NC-2 on MCL cells, and the anti-tumor activity of this receptor were investigated. Main Outcome Measure(s): Flow cytometric analysis and in vitro experiments were performed for showing the activity of NC-2 against cancer. Results: NC-2 receptor was expressed on more than 95% of MCL cells. Pretreatment with D9 monoclonal antibody resulted in about 63% reduction in natural cytotoxicity of MCL cells against WEHI-164 tumor target cells. Conclusions: NC-2 is also one of the receptors expressed on MCL and utilized for WEHI-164 tumor cell killing.

Item Type: Article
Uncontrolled Keywords: monoclonal antibody; natural cytotoxicity; receptor; tumor
Subjects: QZ pathology-Neoplasms
QU Biochemistry > Cell biology and genetics
Divisions: Faculty of Medicine > Basic Sciences Academic Groups > Department of Immunology
Faculty of Medicine > Basic Sciences Academic Groups > Department of Microbiology
Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Depositing User: zahra bagheri .
Date Deposited: 19 Aug 2017 05:52
Last Modified: 19 Aug 2017 05:52
URI: http://eprints.skums.ac.ir/id/eprint/3143

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