Modification of 10 cGy neutron or gamma-rays induced chromosomal damages by hyperthermia: an in vitro study

Fatehi, D. and Mozdarani, H. (2009) Modification of 10 cGy neutron or gamma-rays induced chromosomal damages by hyperthermia: an in vitro study. IRANIAN JOURNAL OF RADIATION RESEARCH, 7 (2). pp. 69-77.

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Abstract

Background: To evaluate the effects of hyperthermia (HT) on the frequency of chromosomal aberrations induced by a low dose of neutron or Y-rays in human peripheral blood lymphocytes. Materials and Methods: Blood samples were exposed to HT (41.5 degrees C for 30 and 60min, 43 degrees C for 15 and 30min), 10 cGy neutron or Y-rays, HT + neutron/Y, and neutron/Y + HT. After standard cell culture, harvesting, fixation and staining, the chromosomal damages were scored in metaphase plates. Results: HT alone at 41.5 degrees C did not induce chromatid or chromosome aberrations, however, the frequency of damages was significantly higher at 43 C (P<0.05). Furthermore, the chromosomal damages was significantly different when cells were irradiated with neutron or Y-rays alone (P<0.01). HT 1 hr post neutron/Y irradiation significantly induced higher chromosome damages in comparison to HT 1 hr before irradiation (P<0.05). The chromosomal damages were remarkably higher when cells were irradiated with neutron then heated at 43 C for 30 min. Conclusion: Since increasing frequency of chromosome damages increases probability of cell death, application of HT after neutron irradiation (instead of X- or gamma- rays) might be considered as a procedure for cells killing in radiotherapy. Iran. J. Radiat. Res., 2009; 7 (2): 69-77

Item Type: Article
Uncontrolled Keywords: Hypeithermia; neutron; gamma rays; chromosomal aberration; cancer
Subjects: QT physiology
QU Biochemistry
QZ pathology-Neoplasms
Divisions: Faculty of Medicine > Basic Sciences Academic Groups > Department of Medical Physics
Depositing User: zahra bagheri .
Date Deposited: 19 Aug 2017 05:38
Last Modified: 19 Aug 2017 05:38
URI: http://eprints.skums.ac.ir/id/eprint/3139

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