Fabrication of an olfactometer for mosquito behavioural studies

Omrani, S.M. and Vatandoost, H. and Oshaghi, M.A. and Shokri, F. and Guerin, P.M. and Yaghoobi Ershadi, M.R. and Rassi, Y. and Tirgari, S. (2010) Fabrication of an olfactometer for mosquito behavioural studies. JOURNAL OF VECTOR BORNE DISEASES, 47 (1). pp. 17-25.

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Abstract

Background & objectives: Olfaction is the major sensory modality involved in the resource searching behaviour of insects including vector mosquitoes (Diptera: Culicidae). To date, our current country-wide knowledge on the host-seeking behaviour of Iranian mosquitoes is mainly confined to host preference which has exclusively come from field studies. Olfactometer is a scientific tool by which more naive aspects of man-vector contact can be clarified under controlled and less biased conditions. Methods: The wind tunnel and stimulus delivery system was constructed from acrylic materials based on previously introduced models with some modifications. Air supply and required light were ensured by a powerful compressor and incandescent bulbs, respectively. Desired level of temperature was maintained by controllable heating radiators. For humidity production a unique in-built piezo system was devised in the course of the air flow. Fine regulators facilitated the continuous generatation of the humidity at a preset level. Results: Titanium tetrachloride smoke plus monitoring of the wind speed revealed that the flow of air was proper and invariable. A desired level of humidity and temperature could be set up in just 10 and 15-45 min, respectively. These physical parameters varied only +/-2% (humidity) and +/-0.15 degrees C (temperature) in a typical 20 min duration. Conclusion: The first sophisticated olfactometer in the field of medical entomology in Iran is reported here. Fast set up and stability of physical parameters are its salient features. It is expected that with the aid of this olfactometer further information on the physiological principles of the host-seeking behaviour of mosquitoes become available soon.

Item Type: Article
Uncontrolled Keywords: Host-seeking; Iran; mosquito; olfactometer; olfactory behaviour
Subjects: W General medicine- Health professions > W.20.55 H9 special topics A-Z (Human expenmentation)
Divisions: Faculty of Medicine > Basic Sciences Academic Groups > Department of Parasitology and Mycology
Depositing User: zahra bagheri .
Date Deposited: 19 Aug 2017 04:15
Last Modified: 19 Aug 2017 04:15
URI: http://eprints.skums.ac.ir/id/eprint/3124

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