Comparison of Real-Time PCR with Disk Diffusion, Agar Screen and E-test Methods for Detection of Methicillin-Resistant Staphylococcus aureus

Shariati, L. and Validi, M. and Tabatabaiefar, M.A. and Karimi, A. and Nafisi, M.R. (2010) Comparison of Real-Time PCR with Disk Diffusion, Agar Screen and E-test Methods for Detection of Methicillin-Resistant Staphylococcus aureus. CURRENT MICROBIOLOGY, 61 (6). pp. 520-524.

[img]
Preview
Text
3.pdf

Download (204kB) | Preview

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is a nosocomial pathogen. Our main objective was to compare oxacillin disk test, oxacillin E-test, and oxacillin agar screen for detection of methicillin resistance in S. aureus, using real-time PCR for mecA as the ``gold standard'' comparison assay. 196 S. aureus isolates were identified out of 284 Staphylococcus isolates. These isolates were screened for MRSA with several methods: disk diffusion, agar screen (6.0 mu g/ml), oxacillin E-test, and real-time PCR for detection of mecA gene. Of the 196 S. aureus isolates tested, 96 isolates (49%) were mecA-positive and 100 isolates (51%) mecA-negative. All methods tested had a statistically significant agreement with real-time PCR. E-test was 100% sensitive and specific for mecA presence. The sensitivity and specificity of oxacillin agar screen method were 98 and 99%, respectively and sensitivity and specificity of oxacillin disk diffusion method were 95 and 93%, respectively. In the present study, oxacillin E-test is proposed as the best phenotypic method. For economic reasons, the oxacillin agar screen method (6.0 mu g/ml), which is suitable for the detection of MRSA, is recommended due to its accuracy and low cost.

Item Type: Article
Subjects: WC Communicable Diseases > Infection.Bacterial Infections
Divisions: Faculty of Medicine > Basic Sciences Academic Groups > Department of Immunology
Faculty of Medicine > Basic Sciences Academic Groups > Department of Microbiology
Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Depositing User: zahra bagheri .
Date Deposited: 19 Aug 2017 03:25
Last Modified: 19 Aug 2017 03:25
URI: http://eprints.skums.ac.ir/id/eprint/3115

Actions (login required)

View Item View Item