Evaluation of in vivo immune response activity and in vitro anti-cancer effect by Scrophularia megalantha

Azadmehr, Abbas. and Hajiaghaee, Reza. and Afshari, Afshin. and Amirghofran, Zahra. and Rafieian-Kopaei, Mahmoud. and Yousofi Darani, Hossein. and shirzad, Hedayatollah. (2011) Evaluation of in vivo immune response activity and in vitro anti-cancer effect by Scrophularia megalantha. JOURNAL OF MEDICINAL PLANTS RESEARCH, 5 (11). pp. 2365-2368.

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Abstract

Many studies have showed that plant extracts possess immunomodulatory and anti-cancer effects. In this study, these effects of Scrophularia megalantha extract, a native plant in Iran, were investigated in vitro and in vivo. Microculture tetrazolium test (MTT assay) was employed to evaluate the effects of S. megalantha on human Jurkat (lymphoblast-like) cell line cytotoxicity. Furthermore, delayed type hypersensitivity (DTH) and hemagglutination tests in mice employed to evaluate the effects of S. megalantha on cellular and humoral responses, respectively. In vitro exposures of the Jurkat cells with various concentrations of S. megalantha extract (0.05, 0.1 and 0.2 mg/mL) significantly suppressed their growth in a dose-dependent manner. The maximum tumor cell growth inhibition was observed with 0.2 mg/mL of the extract. Moreover, the production of specific antibody to sheep red blood cell (SRBC) antigen in immunized mice significantly increased by different concentrations of S. megalantha extract. In conclusion, our results indicated that S. megalantha extract could be an immunomodulator plant and a good candidate for further investigations in order to develop a natural compound as an immunomodulator and anticancer agent.

Item Type: Article
Uncontrolled Keywords: Scrophularia megalantha; extract; tumor growth; immune response
Subjects: QZ pathology-Neoplasms
QV pharmacology > QV 752 Pharmacognosy
Divisions: Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Reserach Vice-Chancellar Department > Medical Plants Research Center
Depositing User: zahra bagheri .
Date Deposited: 16 Aug 2017 04:06
Last Modified: 30 Jan 2018 09:34
URI: http://eprints.skums.ac.ir/id/eprint/3098

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