Effects of selegiline, a monoamine oxidase B inhibitor, on differentiation of P19 embryonal carcinoma stem cells, into neuron-like cells

Bakhshalizadeh, Shabnam. and Esmaeili, Fariba. and Houshmand, Fariba. and shirzad, Hedayatollah. and Saedi-Marghmaleki, Mojtaba. (2011) Effects of selegiline, a monoamine oxidase B inhibitor, on differentiation of P19 embryonal carcinoma stem cells, into neuron-like cells. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 47 (8). pp. 550-557.

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Abstract

Selegiline, the irreversible inhibitor of monoamine oxidase B (MAO-B), is currently used to treat Parkinson's disease. However, the mechanism of action of selegiline is complex and cannot be explained solely by its MAO-B inhibitory action. It stimulates gene expression, as well as expression of a number of mRNAs or proteins in nerve and glial cells. Direct neuroprotective and antiapoptotic actions of selegiline have previously been observed in vitro. Previous studies showed that selegiline can induce neuronal phenotype in cultured bone marrow stem cells and embryonic stem cells. Embryonal carcinoma (EC) cells are developmentaly pluripotene cells which can be differentiated into all cell types under the appropriate conditions. The present study was carried out to examine the effects of selegiline on undifferentiated P19 EC cells. The results showed that selegiline treatment had a dramatic effect on neuronal morphology. It induced the differentiation of EC cells into neuron-like cells in a concentration-dependent manner. The peak response was in a dose of selegiline significantly lower than required for MAO-B inhibition. The differentiated cells were immunoreactive for neuron-specific proteins, synaptophysin, and beta-III tubulin. Stem cell therapy has been considered as an ideal option for the treatment of neurodegenerative diseases. Generation of neurons from stem cells could serve as a source for potential cell therapy. This study suggests the potential use of combined selegiline and stem cell therapy to improve deficits in neurodegenerative diseases.

Item Type: Article
Uncontrolled Keywords: Neuronal differentiation; Embryonic carcinoma cells; Neuron specific markers; Synaptophysin; beta-III tubulin
Subjects: WL Nervous system
QZ pathology-Neoplasms
QU Biochemistry > Cell biology and genetics
Divisions: Faculty of Medicine > Basic Sciences Academic Groups > Department of Physiology
Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Depositing User: zahra bagheri .
Date Deposited: 16 Aug 2017 03:16
Last Modified: 02 Jun 2018 10:02
URI: http://eprints.skums.ac.ir/id/eprint/3085

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