Effect of Allium ampeloprasum on ileum function: Involvement of beta-adrenergic receptors and voltage dependent calcium channels

Sedighi, M. and Rafieian-Kopaei, Mahmoud. and Noori-Ahmadabadi, M. (2012) Effect of Allium ampeloprasum on ileum function: Involvement of beta-adrenergic receptors and voltage dependent calcium channels. LIFE SCIENCE JOURNAL-ACTA ZHENGZHOU UNIVERSITY OVERSEAS EDITION, 9 (4). pp. 1660-1667.

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Abstract

Allium ampeloprasum known as wild leek is a wild nutritious plant that belongs to Lilaceae. In this research, the hypoglycemic effects of the plant's leaves hydro-alcoholic extract on Wistar rat ileum contractions and its possible mechanism have been reviewed. Extraction was done through the maceration of Allium ampeloprasum powder with 70% alcohol. In this intervention research, 48 Wistar rats weighing between 150 and 200 grams were divided into 6 random groups of eight. The groups include: control group, the group receiving Allium ampeloprasum extract cumulative concentrations, the group receiving Proprapanolol, the group receiving Narcan, the group receiving L-name, and the group receiving cumulative concentrations of calcium chloride. On the experiment day, Wistar rats ileum contractions under 1g initial tension were separately recorded through adding potassium chloride 60(mM) in isotonic method in an organ bath containing Tyrode solution (37 degrees C, PH 7.4). To examine the mechanism of the extract effect, the tissue was incubated with Proprapanolol, Narcan or L-name, and the percentage of contraction changes were calculated and recorded. In order to determine the role of calcium channels in the tissue motor activity, ileum affected by calcium chloride cumulative concentrations was used. Allium ampeloprasum cumulative extracts (100, 200, and 400 mg/kg), in a dose-dependent manner, reduced ileum contractions (P<0.0001) by potassium chloride (60 mM). The intervention of beta adrenergic receptor antagonist (Proprapanolol, 1 mu M), opioid receptors (Narcan, 1 mu M), nitric oxide synthase inhibitor (L-name, 100 mu M) in ileum showed that Proprapanolol decreases the inhibitory effects of the extract on the contractions caused by potassium chloride significantly (P<0.0001). However, L-name and Narcan did not decrease the inhibitory effect of the extract on ileum. Calcium also caused the contraction of tissue depolarized by potassium chloride. This contractive effect was significantly decreased by cumulative concentrations of the extract (P<0.0001). It can be concluded that Allium ampeloprasum leaf hydro-alcoholic extract could affect rat ileum motor activity by affecting beta adrenergic receptors and voltage dependent calcium channels. According to the results of the aforementioned effect, it might be used to treat digestive problems. Sedighi M, Rafieian-kopaei., Noori-Ahmadabadi M. Effect of Allium ampeloprasum on ileum function: Involvement of beta-adrenergic receptors and voltage dependent calcium channels. Life Sci J 2012;9(4):1660-1667] (ISSN:1097-8135). http://www.lifesciencesite.com. 255

Item Type: Article
Uncontrolled Keywords: Allium ampeloprasum extract; Ileum; Rat
Subjects: WI Digestive System
QV pharmacology > QV 752 Pharmacognosy
Divisions: Reserach Vice-Chancellar Department > Medical Plants Research Center
Depositing User: zahra bagheri .
Date Deposited: 14 Aug 2017 08:05
Last Modified: 30 May 2018 04:24
URI: http://eprints.skums.ac.ir/id/eprint/2906

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