Comparing the effects of nigella sativa extract and gentamicin in treatment of urinary tract infection caused by Ecoli

Sadri, Seyed Morteza. and Namjoo, Abdolrasoul. and Rafieian-Kopaei, Mahmoud. and Ashrafi, Koorosh. and Shahin Fard, Najmeh. and Ansari Samani, Roya. and Moosavi Azmareh, Fatemeh. (2013) Comparing the effects of nigella sativa extract and gentamicin in treatment of urinary tract infection caused by Ecoli. Journal of Mazandaran University of Medical Sciences, 22 (96). pp. 21-29.


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Background and purpose: The antibiotics' side effects and microbial resistance have increased the need for natural antimicrobial agents in treating infections. This study aimed to investigate the antimicrobial effects of Nigella sativa extract and its possible nephrotoxicity compared with gentamicin. The effect of N. sativa on gentamicin induced renal toxicity and its synergistic effect were evaluated on urinary tract infection caused by Ecoli in rabbits. Materials and methods: In this experimental study, 36 male New Zealand rabbits were designated into seven groups: gentamicin-bacteria, N. sativa-bacteria, N. sativa-gentamicin-bacteria, N. sativa-gentamicin, bacteria, gentamicin and control groups. The animals were anesthetized after ten days of treatment, and the kidney specimens were collated for histopathological examination. The nephrotoxic effects of gentamicin and protective effects of N. sativa on kidney were studied. Antibacterial effects of the extracts were evaluated with laboratory tests and the MBC and MIC values were obtained for N. sativa. Results: The level of urea nitrogen and creatinine in urine increased in bacteria group compared to control group (P<0.05). But, they decreased in bacteria- N. sativa group compared with the bacterial group (P<0.05). Histopathological examination of kidney tissue showed that renal lesions in bacterial and, bacteria- gentamicin groups (ATN) were more than N. sativa -bacteria and bacteria-gentamycin- N. sativa (minor necrosis) groups. Conclusion: According to the results, N. sativa in addition to antibacterial effect against E. coli, can prevent the nephrotoxic effects of gentamicin. Therefore, it may be considered as an alternative, or in combination with gentamicin.

Item Type: Article
Additional Information: cited By 1
Uncontrolled Keywords: creatinine; gentamicin; Nigella sativa extract, animal experiment; animal tissue; antibacterial activity; article; black cumin; controlled study; Escherichia coli; histopathology; kidney injury; male; minimum inhibitory concentration; nephrotoxicity; nonhuman; rabbit; urea nitrogen blood level
Subjects: WC Communicable Diseases > Infection.Bacterial Infections
WJ Urogenital System
QV pharmacology > QV 752 Pharmacognosy
Divisions: Faculty of Medicine > Department of Clinical Sciences > Department of Pathology
Reserach Vice-Chancellar Department > Medical Plants Research Center
Depositing User: Users 1 not found.
Date Deposited: 09 Aug 2017 06:46
Last Modified: 24 Jul 2018 04:38

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