Toll like receptor 2 and 4 expression in peripheral blood mononuclear cells of multiple sclerosis patients

Hasheminia, Seyed Javad. and Zarkesh Esfahani, Hamid. and Tolouei, Sepideh. and Shaygannejad, Vahid. and shirzad, Hedayatollah. and Hashemzadeh-Chaleshtori, Morteza. (2014) Toll like receptor 2 and 4 expression in peripheral blood mononuclear cells of multiple sclerosis patients. Iranian Journal of Immunology, 11 (2). pp. 74-83.


Download (665kB) | Preview
Official URL:


Background: Multiple sclerosis (MS) is a T cell mediated autoimmune disease with unknown etiology. Appropriate MS therapeutic strategies need thorough understanding of both disease etiology and pathogenesis mechanisms. Ligation of TLR-2 and TLR-4 stimulates the production of several cytokines leading to CNS autoimmunity and neurodegenerative diseases. Objective: To find a relationship between MS disability and TLR-2 and TLR-4 expression on mononuclear cells in the blood of MS patients. Methods: Forty-five new case (NC) MS patients (33 females and 12 males) and 45 age and gender-matched healthy controls (HC) were recruited to the study. PBMCs were prepared and the expressions of TLR-2 and TLR-4 were assessed by flowcytometry technique using appropriate monoclonal antibodies. Results: Our results showed that the expression of TLR-2 and TLR-4 proteins in the patients group was significantly higher than that of healthy controls. TLR-2 but not TLR-4 was correlated with expanded disability status scale (EDSS) scores. Conclusion: High expressions of TLR-2 and TLR-4 may represent a state of innate immune activation in patients with MS. © 2014, Shiraz University of Medical Sciences.

Item Type: Article
Additional Information: cited By 6
Uncontrolled Keywords: toll like receptor 2; toll like receptor 4; toll like receptor 2; toll like receptor 4, adolescent; Article; clinical article; controlled study; Expanded Disability Status Scale; female; flow cytometry; human; human cell; innate immunity; male; multiple sclerosis; peripheral blood mononuclear cell; protein expression; adult; case control study; disability; gene expression; genetics; immunophenotyping; metabolism; middle aged; mononuclear cell; multiple sclerosis; severity of illness index; young adult, Adolescent; Adult; Case-Control Studies; Disability Evaluation; Female; Gene Expression; Humans; Immunophenotyping; Leukocytes, Mononuclear; Male; Middle Aged; Multiple Sclerosis; Severity of Illness Index; Toll-Like Receptor 2; Toll-Like Receptor 4; Young Adult
Subjects: WL Nervous system
QU Biochemistry > Cell biology and genetics
Divisions: Faculty of Medicine > Basic Sciences Academic Groups > Department of Immunology
Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Depositing User: zahra bagheri .
Date Deposited: 05 Aug 2017 05:43
Last Modified: 05 Feb 2018 07:19

Actions (login required)

View Item View Item