Antimicrobial activity of endophytic bacterial populations isolated from medical plants of Iran

Beiranvand, Maryam. and Amin, Mansour. and Hashemi-Shahraki, Abdolrazag. and Romani, Bizhan. and Yaghoubi, Sajad. and Sadeghi, Parisa. (2017) Antimicrobial activity of endophytic bacterial populations isolated from medical plants of Iran. Iranian Journal of Microbiology, 9 (1). pp. 11-18.


Download (665kB) | Preview
Official URL:


Endophytic actinobacteria colonize inside the plant tissues without causing damages to the host plant. Since these microorganisms colonize in the different parts of plants and can stop plant disease, they have been applied as biological agents for controlling human diseases. The aim of this study was molecular identification and measuring the antimicrobial activity of endophytic Actinomycetes isolated from medicinal plants of Iran. Materials and Methods: The total of 23 medicinal plant samples were collected, sterilized, and crushed. Small pieces of the crushed samples were then cultured directly on three selective media. Grown colonies were identified by 16S rRNA gene sequencing method. Each isolate was cultured in TSB medium and then antimicrobial compound was extracted using ethyl acetate and tested against the target bacteria. Results: Sixteen out of 23 bacterial isolates (69%) exhibited antimicrobial activity against the selected pathogenic bacteria, such as Bacillus cereus, Staphylococcus aureus, Bacillus subtilis, Klebsiella pneumoniae, Citrobacter freundii, Proteus mi-rabilis, Shigella flexneri and Escherichia coli. Conclusion: Our Study showed a high phylogenetic diversity and the potent antibiotic activity of endophytic bacteria in medicinal plants of Iran. © 2017, Tehran University of Medical Science. All rights reserved.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: Actinomycets, Antimicrobial, Endophytic, Medicinal plants
Subjects: QV pharmacology > QV 704 Pharmaceutics
Divisions: Reserach Vice-Chancellar Department > Medical Plants Research Center
Depositing User: Users 1 not found.
Date Deposited: 16 Jun 2017 16:03
Last Modified: 24 Apr 2018 07:13

Actions (login required)

View Item View Item