Effect of flavonoids of extract of cichorium intybus L. leaf on induction of P19 stem-cells differentiation to insulin-producing cells

Ebrahiminia, M. and Esmaeili, F. and Kharazian, N. and Houshmand, F. and Ebrahimi, M. (2014) Effect of flavonoids of extract of cichorium intybus L. leaf on induction of P19 stem-cells differentiation to insulin-producing cells. Journal of Mazandaran University of Medical Sciences, 23 (110). pp. 92-100.

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Abstract

Background and purpose: Regarding the effective role of plant flavonoids in the prevention and treatment of diabetes mellitus, in this investigation, induction of P19 stem cells differentiation into insulin producing cells by flavonoids of methanol extract of Cichorium intybus L. leaf was examined. Materials and methods: Initially, flavonoids of methanol extract of Cichorium intybus L. leaf were concentrated. The P19-derived embryonic bodies were cultured in α-MEM (Minimum Essential Medium) containing 15 fetal bovine serum for three days. To induce the differentiation, the cells treated with the concentrations of 50, 100 and 200 μg/ml of flavonoids of methanol extract of Cichorium intybus L. leaf for 12 days. Untreated P19 cells and embryonic bodies were used as controls. Dithizone staining, immunoflorecence and real-time polymerase chain reaction (RT-PCR) techniques were used to demonstrate the differentiated cells. Results: Differentiated P19 cells by different concentrations of extract shown positive reaction to dithizone staining. Most percentage of positive dithizone cells was reached in concentration of 100 μg/ml. Immunoflorecence method showed that differentiated P19 cells were able to express the pancreas beta cell-specific markers. Pdx-1 gene expression in the cells was demonstrated by RT-PCR technique. Conclusion: The flavonoids of methanol extract of Cichorium intybus L. leaf are able to induce differentiation of P19 stem cells to insulin producing cells. © 2014 Mazandaran University of Medical Sciences. All rights reserved.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: bovine serum albumin; chicory leaf extract; dithizone; flavonoid; insulin; plant extract; transcription factor PDX 1; unclassified drug, Article; cell differentiation; chicory; controlled study; diabetes mellitus; embryoid body; embryonal carcinoma stem cell; gene expression; immunofluorescence test; nonhuman; P19 stem cell; pancreas islet beta cell; real time polymerase chain reaction
Subjects: QU Biochemistry > Cell biology and genetics
QV pharmacology > QV 752 Pharmacognosy
Divisions: Faculty of Medicine > Basic Sciences Academic Groups > Department of Physiology
Depositing User: zahra bagheri .
Date Deposited: 02 Aug 2017 06:24
Last Modified: 02 Aug 2017 06:24
URI: http://eprints.skums.ac.ir/id/eprint/2237

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