Effect of pterostilbene on cellular proliferation inhibition and induction of apoptosis in lymphoblastic leukemia cell line

Rahimnejad, Tahereh. and Beshkar, Pejman. and shirzad, Hedayatollah. and Rafieian-Kopaei, Mahmoud. and Safdari, Vahid. and Asgarian Dehkordi, Narges. and Pourgheysari, Batoul. (2014) Effect of pterostilbene on cellular proliferation inhibition and induction of apoptosis in lymphoblastic leukemia cell line. Journal of Babol University of Medical Sciences, 16 (12). pp. 32-38.

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Abstract

BACKGROUND AND OBJECTIVE: Acute lymphoblastic leukemia (ALL=Acule Lymphoblastic Leukemia) is the most common type of hematological malignancies in Iranian children. Pterostilbene (PT) is a natural stilbenoid in blueberries. Since the effect of PT on lymphoblastic leukemia cell line has not been investigated, the aim of the present study was to evaluate the effects of PT induction and cellular proliferation inhibition on this cell line. METHODS: In this experimental study, Jurkat cell line was cultured in standard conditions with different concentrations of PT (0, 30, 40, 50, 60, 80 and 100 Dm) for 24, 48 and 72 hours and compared with the cell of control group. Then, the effect of PT on cell viability and induction of apoptosis was measured using MTS assay and Annexin V-FITC kit. FINDINGS: Inhibition of cellular proliferation was 44, 47 and 57 at 60 Dm concentration and 71, 78 and 89 at 100 Dm concentration after 24, 48 and 72 hours of PT incubation, respectively (p<0.05). Cell apoptosis was 60 at 80 Dm, 50 at 60 Dm and 27 at 40 Dm compared to 4.63 in control. There was significant difference at 80 Dm concentration. CONCLUSION: The results demonstrated that the PT was effective on the proliferation inhibition and induction of apoptosis in Jurkat lymphoblastic leukemia cell line. The study of the mechanism of apoptosis induction by using this combination can be a progressive step into target therapy. © 2014 Babol University of Medical Sciences. All rights reserved.

Item Type: Article
Additional Information: cited By 1
Uncontrolled Keywords: pterostilbene; stilbene derivative; unclassified drug, apoptosis; Article; cell proliferation; cell viability; controlled study; experimental study; human; human cell; jurkat cell line; lymphatic leukemia; MTS assay
Subjects: WH Hemic and Lymphatic System
WS Pediatrics
QU Biochemistry
Divisions: Faculty of Medicine > Basic Sciences Academic Groups > Department of Biochemistry and Genetics
Faculty of Medicine > Basic Sciences Academic Groups > Department of Immunology
Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Reserach Vice-Chancellar Department > Medical Plants Research Center
Depositing User: zahra bagheri .
Date Deposited: 02 Aug 2017 05:45
Last Modified: 17 Apr 2018 07:08
URI: http://eprints.skums.ac.ir/id/eprint/2231

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