The effect of adiponectin on matrix metalloproteinase-9 (MMP-9) in vascular smooth muscle cells

Saneipour, Maryam. and Gatreh Samani, Keihan. and Heydarian, Esfandiar and Farrokhi, Effat. and Abdian, Narges. (2014) The effect of adiponectin on matrix metalloproteinase-9 (MMP-9) in vascular smooth muscle cells. Journal of Kerman University of Medical Sciences, 21 (5). pp. 384-393.

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Abstract

Background & Aims: Atherosclerosis is a major cause of morbidity and mortality. Adiponectin reduces the risk of heart disease, and matrix metalloproteinase-9 (MMP-9) is involved in the formation and development of atherosclerotic plaque. The aim of this study was the investigation of the effect of adiponectin on MMP-9 gene expression. It seems this hormone can reduce the risk of atherosclerosis by MMP-9 gene expression reduction. Methods: Human aorta smooth muscle cells were cultured in F12K media in appropriate environment and conditions, then, the cells were treated with 5 µg/ml adiponectin. After 24 and 48 hour, total RNA was extracted and corresponding cDNA was made. After drawing standard curve and determining the efficiency of the reaction, MMP-9 gene expression was measured by the SYBR Green kit and real time PCR method. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene was the reference gene. The amount of MMP-9 protein, compared to the β-actin protein as reference protein, was estimated with Western blot method. Results: adiponectin did not cause a ch ange in gene expression of MMP-9 in 24 hours, but in 48 hours reduced gene expression (-1.1, and -5.5, respectively). As a result of MMP-9 gene expression reduction, protein also reduced after 48 hours compared to β-actin protein. Conclusion: Through the reduction of MMP-9 protein and gene expression, adiponectin changes extra cellular matrix which may reduce the risk and complications of atherosclerosis. © 2014, Kerman University of Medical Sciences. All rights reserved.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: adiponectin; beta actin; gelatinase B; glyceraldehyde 3 phosphate dehydrogenase, aorta media; Article; atherosclerosis; cell culture; controlled study; extracellular matrix; gene expression; human; human cell; RNA extraction; vascular smooth muscle; Western blotting
Subjects: WG Cardiovascular System
QU Biochemistry
Divisions: Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Reserach Vice-Chancellar Department > Clinical Biochemistry Research Center
Depositing User: zahra bagheri .
Date Deposited: 01 Aug 2017 05:32
Last Modified: 21 May 2018 03:52
URI: http://eprints.skums.ac.ir/id/eprint/2171

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