Development of an indirect sandwich ELISA for detection of urinary antigen, using Legionella pneumophila PAL protein

Gholipour, A. and Moosavian, M. and Makvandi, M. and Galehdari, H. and Alvandi, A. and Mard, S.A. (2014) Development of an indirect sandwich ELISA for detection of urinary antigen, using Legionella pneumophila PAL protein. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 30 (5). pp. 1463-1471.

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Abstract

Legionella pneumophila peptidoglycan-associated lipoprotein (PAL) protein is an extremely conserved antigen among Legionella species. In this study, rabbit and rat anti-PAL immunoglobulin G antibodies were produced by immunization with purified, recombinant PAL (r-PAL) protein of L. pneumophila serogroup 1 and used as capture and detection antibodies in the PAL antigen-based enzyme-linked immunosorbent assay (ELISA) to detect urinary PAL antigen. Urine samples were obtained from rats experimentally infected with L. pneumophila serogroup 1. The PAL antigen was measured in urine samples of 40 infected and 40 uninfected rats. After choosing the cut-off value of 0.192, the sensitivity and specificity of the PAL antigen-based ELISA were 87.5 and 97.5 %, respectively. The results obtained by PAL antigen base ELISA were compared with those obtained by Biotest. The PAL antigen was detected efficiently by both of the assays and all of the control human urine samples were negative by the ELISA test. The PAL antigen-based ELISA assay was relatively simple to perform, precise, highly sensitive and specific, and reproducible. Based on our data the PAL antigen-based ELISA described here is the first indirect sandwich ELISA for urinary antigen detection which could easily be applied for diagnosis of Legionnaires disease.

Item Type: Article
Uncontrolled Keywords: Legionella; Legionnaires disease (LD); Peptidoglycan-associated lipoprotein (PAL); ELISA; EIA
Subjects: WJ Urogenital System
QU Biochemistry
Divisions: Faculty of Medicine > Basic Sciences Academic Groups > Department of Immunology
Faculty of Medicine > Basic Sciences Academic Groups > Department of Microbiology
Depositing User: zahra bagheri .
Date Deposited: 31 Jul 2017 05:13
Last Modified: 31 Jul 2017 05:13
URI: http://eprints.skums.ac.ir/id/eprint/2102

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