Associations of a TLR4 single-nucleotide polymorphism with H. pylori associated gastric diseases in iranian patients

Bagheri, Nader. and Azadegan-Dehkordi, Fatemeh. and Sanei, Hossein. and Taghikhani, Afshin. and Rahimian, Ghorbanali. and Salimzadeh, Loghman. and Hashemzadeh-Chaleshtori, Morteza. and Rafieian-Kopaei, Mahmoud. and Shirzad, Mahsa. and shirzad, Hedayatollah. (2014) Associations of a TLR4 single-nucleotide polymorphism with H. pylori associated gastric diseases in iranian patients. CLINICS AND RESEARCH IN HEPATOLOGY AND GASTROENTEROLOGY, 38 (3). pp. 366-371.

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Objective: Helicobacter pylori (H. pylori) is associated with gastric ulcer and gastric adenocarcinoma. Polymorphisms in the host genes coding for toll-like receptors (TLRs) may influence the innate and adaptive immune response to the infection, affecting the susceptibility to H. pylori or the disease outcomes. But the details and association to different polymorphisms and different clinical expressions in patients infected with H. pylori (different clinical expression of H. pylori infection) remain unclear. Methods: A case-control study consisting of 195 patients with H. pylori infection and 241 H. pylori uninfection was conducted. Genomic DNA was extracted and genotypes of TLR4Asp299Gly polymorphism were assessed through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Presence of cagA was evaluated using PCR. Results: TLR4 (Asp299Gly) G and DG alleles frequency in H. pylori infected population was significantly higher in the chronic gastritis group than in the chronic active gastritis group (P = 0.021; OR, 2.409; 95% CI, 1.124-5.162). Grade mononuclear (MN) infiltration in H. pylori infected patients with DG genotype of TLR-4 Asp299Gly increased significantly. CagA positivity was more frequently associated with chronic active gastritis (P = 0.017, OR = 2.26, 95% CI = 1.144-4.462) and grade polymorphonucler (PMN) infiltration. Conclusion: TLR-4 Asp299Gly G allele substitution may be modified pattern of immune response in the gastric mucosa of H. pylori infected patients and may be H. pylori infected patients with gastritis have increased risk for the development of chronic gastritis. CagA positivity may be arisk factor for development of gastritis. (C) 2013 Elsevier Masson SAS. All rights reserved.

Item Type: Article
Subjects: WI Digestive System
QU Biochemistry
Divisions: Faculty of Medicine > Department of Clinical Sciences > Department of Internal Medicine
Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Reserach Vice-Chancellar Department > Medical Plants Research Center
Depositing User: Users 1 not found.
Date Deposited: 31 Jul 2017 04:01
Last Modified: 30 Jan 2018 08:58

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