New insight to IL-23/IL-17 axis in iranian infected adult patients with gastritis : Effects of genes polymorphisms on expression of cytokines

shirzad, Hedayatollah. and Bagheri, Nader. and Azadegan-Dehkordi, Fatemeh. and Zamanzad, Behnam. and Izadpanah, Esmail. and Abdi, Mohammad. and Ramazani, Ghasem. and Sanei, Mohammad Hossein. and Ayoubian, Hiresh. and Ahmadi, Abbas. and Jamalzehi, Sirous. and Aslani, Parya. and Zandi, Farid. (2015) New insight to IL-23/IL-17 axis in iranian infected adult patients with gastritis : Effects of genes polymorphisms on expression of cytokines. Acta Gastro-Enterologica Belgica, 78 (2). pp. 212-218.

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Abstract

Chronic inflammation is the hallmark of the pathogenesis of H. pylori-induced gastric cancer. IL-17A and IL-17F are inflammatory cytokines expressed by a novel subset of CD4+Th cells and play critical function in inflammation. We evaluated the relationship between IL-17A G197A, IL-17F A7488G and IL23R+2199 A/C polymorphisms with IL-6, IL-17, IL-21, IL-23 and TGF-β1 mRNAs expression in regard to H. pylori infection with chronic gastritis. Total RNA and genomic DNA were extracted from gastric biopsies of 58 H. pylori-infected patient with gastritis. Afterward, mucosal IL-6, IL-17, IL-21, IL-23 and TGF-β1 mRNAs expression and polymorphisms in IL-17A G197A, IL-17F A7488G and IL-23R +2199A/Cin gastric biopsies were determined by real-time PCR and PCR-RFLP. Our results show that IL-17A G197A, IL-17F A7488G andIL23R +2199A/C polymorphisms have no effect on mucosal expression of IL-6, IL-17, IL-21 and TGF-β1 mRNAs expression in H. pylori-infected patients with chronic gastritis. These results suggest that IL-17A G197A, IL-17F A7488G and IL23R +2199A/C polymorphisms no alter mucosal cytokine pattern in Iranian patients with H. pylori-associated gastritis diseases. © Acta Gastro-Enterologica Belgica.

Item Type: Article
Additional Information: cited By 11
Uncontrolled Keywords: beta actin; genomic DNA; interleukin 17; interleukin 17F; interleukin 21; interleukin 23; interleukin 23 receptor; interleukin 6; messenger RNA; transforming growth factor beta1; interleukin derivative; messenger RNA; transforming growth factor beta1, adult; Article; chronic gastritis; DNA extraction; DNA polymorphism; female; gene expression; Helicobacter infection; human; human tissue; Iranian (citizen); major clinical study; male; real time polymerase chain reaction; restriction fragment length polymorphism; RNA extraction; stomach biopsy; gastric mucosa; gastritis; genetic polymorphism; genetics; Helicobacter Infections; Helicobacter pylori; Iran; metabolism; microbiology; middle aged, Adult; Female; Gastric Mucosa; Gastritis; Helicobacter Infections; Helicobacter pylori; Humans; Interleukins; Iran; Male; Middle Aged; Polymorphism, Genetic; RNA, Messenger; Transforming Growth Factor beta1
Subjects: WI Digestive System
QU Biochemistry > Cell biology and genetics
Divisions: Faculty of Nursing and Midwifery
Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Depositing User: Users 1 not found.
Date Deposited: 29 Jul 2017 09:38
Last Modified: 04 Feb 2018 06:07
URI: http://eprints.skums.ac.ir/id/eprint/2038

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