Cytokine gene expression in newly diagnosed multiple sclerosis patients

Hasheminia, Seyed Javad. and Tolouei, Sepideh. and Zarkesh Esfahani, Hamid. and Shaygannejad, Vahid. and shirzad, Hedayatollah. and Torabi, Reza. and Hashemzadeh-Chaleshtori, Morteza. (2015) Cytokine gene expression in newly diagnosed multiple sclerosis patients. Iranian Journal of Allergy, Asthma and Immunology, 14 (2). pp. 208-216.

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Abstract

Multiple Sclerosis (MS) is characterized by multiple areas of inflammation, demyelination and neurodegeneration. Infiltrating Th1 CD4+ T cells secrete proinflammatory cytokines. They stimulate the release of some cytokines, expression of adhesion molecules and these cytokines may cause damage to the myelin sheath and axons. In this study, we analyzed plasma levels and gene expressions of five important cytokines in the new diagnosed MS Patients by ELISA and Real time PCR. PCR amplifications were performed to determine the IL-17, IL-23, IL-10, IL-27 and TGF-β mRNA expression levels using the SYBR Green PCR Kit. Our results showed significant decrease in IL-10, IL-27 and TGF-β but there was no significant difference in the IL-17 and IL-23 between patients and healthy controls. Altogether, our results indicated that dysregulation of cytokines, mainly increased expression of pro-inflammatory cytokines and decreased expression of inhibitory cytokines occurred in MS patients. This study may shed light to the probable role of these cytokines in neurodegeneration mechanism and current or future use of cytokines in managing and treatment of multiple sclerosis.

Item Type: Article
Additional Information: cited By 3
Uncontrolled Keywords: cytokine; interleukin 10; interleukin 17; interleukin 23; interleukin 27; messenger RNA; transforming growth factor beta; cytokine; transcriptome, adult; Article; blood level; clinical article; controlled study; enzyme linked immunosorbent assay; female; gene expression; human; male; multiple sclerosis; nuclear magnetic resonance imaging; real time polymerase chain reaction; biosynthesis; immunology; multiple sclerosis, Adult; Cytokines; Enzyme-Linked Immunosorbent Assay; Female; Humans; Male; Multiple Sclerosis; Real-Time Polymerase Chain Reaction; Transcriptome
Subjects: WL Nervous system
QU Biochemistry > Cell biology and genetics
Divisions: Faculty of Medicine > Basic Sciences Academic Groups > Department of Immunology
Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Depositing User: zahra bagheri .
Date Deposited: 29 Jul 2017 08:52
Last Modified: 05 Feb 2018 07:17
URI: http://eprints.skums.ac.ir/id/eprint/1991

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