The Association of Interleukin-16 Gene Polymorphisms with IL-16 Serum Levels and Risk of Multiple Sclerosis

Farrokhi, M. and Masoudifar, A. and Derakhshan, A. and Saadatmand, S. and Rouhi-Boroujeni, H. and Etemadifar, M. and Rezaei-Zarji, S. and Javid, A. and Nobakht, R. and Deyhimi, M. and Ekramnia, A. and Ebrahimi, M. and Sheikh, S. and Ansaripour, S. and Amani-Beni, A. and Jahanbani-Ardakani, H. (2017) The Association of Interleukin-16 Gene Polymorphisms with IL-16 Serum Levels and Risk of Multiple Sclerosis. Immunological Investigations. pp. 1-9.

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Abstract

Multiple sclerosis (MS) is a chronic inflammatory, demyelinating, and neurodegenerative disease of the central nervous system (CNS) that is immunologically mediated in genetically susceptible individuals. Interleukin-16 (IL-16), a pleiotropic cytokine, is an important regulator of T-cell activation which plays a key role in autoimmune diseases. Single-nucleotide polymorphisms (SNPs) in the IL-16 gene may lead to altered cytokine expression or biological activity, and these variations may modulate an individual’s risk for MS. To test this hypothesis, we investigated association of IL-16 gene SNPs (i.e., rs4072111 C/T, rs11556218 G/T, and rs4778889 C/T) and serum IL-16 levels with risk of MS in an Iranian population. Methods: We analyzed the three SNPs of IL-16 in 250 MS patients and 400 healthy controls using polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP). The serum level of IL-16 was assessed by enzyme-linked immunosorbent assay (ELISA). Results: The IL-16 rs4072111C/T genotype and allele frequencies showed significantly differences between MS patients and controls (p < 0.01). Statistically significant differences were also found in allele and genotype frequencies of rs11556218 G/T between two groups (p ≤ 0.01). The mean serum levels of IL-16 in MS patients were significantly higher in MS patients compared to healthy controls (p ≤ 0.01). Conclusion: In summary, the present study provides the first evidence that the rs11556218T/G and rs4072111C/T polymorphisms of IL-16 gene were significantly associated with increased risk of MS. These results suggest that rs11556218T/G, rs4072111C/T, and rs4778889T/C polymorphisms of IL-16 may contribute to susceptibility to MS through increased expression of IL-16 levels.

Item Type: Article
Additional Information: cited By 0; Article in Press
Uncontrolled Keywords: ELISA,interleukin-16, multiple sclerosis, polymerase chain reaction, polymorphism
Subjects: WL Nervous system
Divisions: Reserach Vice-Chancellar Department > Medical Plants Research Center
Depositing User: zahra bagheri .
Date Deposited: 24 Jun 2017 04:27
Last Modified: 24 Jun 2017 04:27
URI: http://eprints.skums.ac.ir/id/eprint/192

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