Effect of quercetin on methotrexate-induced hepatic and renal damages in male rats

Moradi, Elham. and Heidarian, Esfandiar. and Gholami-Arjenaki, Mostafa. and Saffari-Chaleshtori, Javad. and Mardani, Gashtasb. and Karimi-Taghanaki, Alireza. and Normohammadian, Zahra. (2015) Effect of quercetin on methotrexate-induced hepatic and renal damages in male rats. Journal of Mazandaran University of Medical Sciences, 25 (127). pp. 25-37.

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Abstract

Abstract Background and purpose: Methotrexate as a chemotherapy drug causes chronic liver damage, infiltration of neutrophils, oxidative stress, and direct renal tubular damage. Quercetin is a flavonoid with antioxidant and anti-inflammatory properties. Therefore, the aim of this study was to investigate the effect of quercetin on eliminating the liver and kidney toxicity of methotrexate. Materials and methods: In this experimental study, 32 rats were divided into 4 groups. Group I (control) was given regular diet. Group II received single-dose methotrexate. Group III received methotrexate + a single dose quercetin and the last group (positive control) received methotrexate + a single dose silymarin. After five days, blood samples were taken and the serum GOT, GPT, ALP, Cr, urea and antioxidant capacity of plasma were measured. Some parts of liver and kidney were removed to measure the liver and kidney SOD, MDA, catalase activity and histopathological studies. Results: Serum GOT, GPT, ALP, Cr, and liver and kidney MDA were significantly higher (P<0.05) in group II, compared with those of the control group. These parameters significantly decreased (P<0.05) in group III. Compared to the control group, antioxidant capacity of plasma, activity of the liver and kidney SOD, catalase and serum urea decreased significantly in group II (P<0.05). Administration of quercetin significantly increased these parameters (P<0.05) and decreased hepatic and renal lymphocyte infiltration. Conclusion: According to the results, administration of quercetin could have a protective role in preventing liver and renal toxicity induced by methotrexate which could be due to its antioxidant property.

Item Type: Article
Additional Information: cited By 1
Uncontrolled Keywords: alanine aminotransferase; alkaline phosphatase; aspartate aminotransferase; catalase; creatinine; malonaldehyde; methotrexate; quercetin; silymarin; superoxide dismutase; urea, animal experiment; animal model; animal tissue; antioxidant activity; Article; controlled study; drug effect; histopathology; kidney injury; liver injury; lymphocytic infiltration; male; nonhuman; rat; single drug dose
Subjects: WJ Urogenital System
WK Endocrine System
QV pharmacology > QV 704 Pharmaceutics
Divisions: Faculty of Health > Department of Environmental Health
Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Reserach Vice-Chancellar Department > Clinical Biochemistry Research Center
Reserach Vice-Chancellar Department > Medical Plants Research Center
Depositing User: zahra bagheri .
Date Deposited: 29 Jul 2017 05:15
Last Modified: 12 Jun 2018 07:11
URI: http://eprints.skums.ac.ir/id/eprint/1910

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