Genotypic and phenotypic characteristics of vancomycin-resistant enterococcus isolated from clinical specimens of patients in shahrekord

Abbasi, Safieh. and Zamanzad, Behnam. (2015) Genotypic and phenotypic characteristics of vancomycin-resistant enterococcus isolated from clinical specimens of patients in shahrekord. Journal of Mazandaran University of Medical Sciences, 24 (122). pp. 98-106.

[img]
Preview
Text
11.pdf

Download (233kB) | Preview
Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Background and purpose: Enterococci are important agents of endocarditis and urinary tract infections. They acquire resistance against antimicrobial agents which causes difficulty in their treatment. This study aimed to determine the antimicrobial susceptibility of strains resistant to vancomycin and identification of resistance genes vanA and vanB Enterococcus strains isolated from teaching hospitals in 2014. Material and methods: Hundred and fifty isolates of Enterococcus were isolated from hospitals and the genus and species composition were identified using different biochemical methods. Antibiogram tests were also conducted. The minimum inhibitory concentration was conducted applying broth micro dilution. Finally the strains containing vanA and VanB genes were identified. Results: The antibiogram test showed 10 strains with VanA and VanB phenotype. All strains were resistant to ciprofloxacin. VanA and VanB genes were detected in all 10 isolates of vanA phenotype and two strains of vanB phenotype. VanA and VanB genes were proliferated by PCR in two strains of the10 strains that were VanA phenotype. Conclusion: Among Enterococcus species E. faecium and E. faecalis isolates were detected in teaching hospitals. The highest rate of resistance was to erythromycin and tetracycline and the lowest resistance rate was to vancomycin.

Item Type: Article
Additional Information: cited By 1
Uncontrolled Keywords: ciprofloxacin; erythromycin; tetracycline; vancomycin, antibiotic resistance; antibiotic sensitivity; Article; bacterial gene; bacterium detection; bacterium isolate; broth dilution; Enterococcus faecium; minimum inhibitory concentration; nonhuman; phenotype; polymerase chain reaction; teaching hospital; vanA gene; vanB gene; vancomycin resistant Enterococcus
Subjects: WC Communicable Diseases > Infection.Bacterial Infections
QU Biochemistry > Cell biology and genetics
Divisions: Faculty of Medicine > Basic Sciences Academic Groups > Department of Immunology
Faculty of Medicine > Basic Sciences Academic Groups > Department of Microbiology
Depositing User: Users 1 not found.
Date Deposited: 29 Jul 2017 05:07
Last Modified: 06 Feb 2018 10:36
URI: http://eprints.skums.ac.ir/id/eprint/1905

Actions (login required)

View Item View Item