Effects of Feeder Layers, Culture Media, Conditional Media, Growth Factors, and Passages Number on Stem Cell Optimization

Ghasemi-Dehkordi, Payam. and Allahbakhshian-Farsani, Mehdi. and Abdian, Narges. and Mirzaeian, Amin. and Hashemzadeh-Chaleshtori, Morteza. and Jafari-Ghahfarokhi, Hamideh. (2015) Effects of Feeder Layers, Culture Media, Conditional Media, Growth Factors, and Passages Number on Stem Cell Optimization. Proceedings of the National Academy of Sciences India Section B - Biological Sciences, 85 (3). pp. 711-717.


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Stem cells are undifferentiated and self-renewal cells which could be obtained from the body or artificially derived from an adult somatic cell by forced expression of specific genes. In recent years stem cells are widely used in laboratory for tissue engineering and therapeutic applications. There are different factors and conditions that affect the stem cell culture such as feeder layers, atmosphere, kind of medium, growth factors, passages number, and conditional media with animal or human sources. Optimization of stem cell culture for medical approaches and regenerative medicine is important. Therefore, in the present study, the effect of these factors and agents on optimization of stem cell culture has been discussed. This review study showed that optimization of feeder layer, atmosphere, and using supplemented media with essential growth factors could help in maintaining the stem cells in undifferentiated state in vitro. The present study indicated that optimization of stem cell culture depends on the kind of each cell type and using stem cells in low passage number could decrease chromosomal abnormalities and DNA damages. For inhibiting the stem cell contamination by feeder cell lines, culture of these cells on feeder free systems like Matrigel matrix in conditioned media supplemented with essential growth factors is useful. Also, for eradicating immune system responses and reducing the risk of animal pathogen transfer, culture of stem cells on human feeder in optimized media is suitable for therapeutic approaches and regenerative medicine.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: Stem cell Feeder layer Atmosphere Culture media Conditional media Growth factor Passages number Intr
Subjects: QU Biochemistry > Cell biology and genetics
Divisions: Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Reserach Vice-Chancellar Department > Clinical Biochemistry Research Center
Depositing User: Users 1 not found.
Date Deposited: 26 Jul 2017 09:45
Last Modified: 03 Mar 2018 08:03
URI: http://eprints.skums.ac.ir/id/eprint/1861

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