Production of stable GFP-expressing neural cells from P19 embryonal carcinoma stem cells

shirzad, Hedayatollah. and Esmaeili, Fariba. and Bakhshalizadeh, Shabnam. and Ebrahimie, Marzieh. and Ebrahimie, Esmaeil. (2017) Production of stable GFP-expressing neural cells from P19 embryonal carcinoma stem cells. Molecular and Cellular Probes, 32. pp. 46-54.

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Abstract

Murine P19 embryonal carcinoma (EC) cells are convenient to differentiate into all germ layer derivatives. One of the advantages of P19 cells is that the exogenous DNA can be easily inserted into them. Here, at the first part of this study, we generated stable GFP-expressing P19 cells (P19-GFP+). FACS and western-blot analysis confirmed stable expression of GFP in the cells. We previously demonstrated the efficient induction of neuronal differentiation from mouse ES and EC cells by application of a neuroprotective drug, selegiline In the second part of this study selegiline was used to induce differentiation of P19-GFP+ into stable GFP-expressing neuron-like cells. Cresyl violet staining confirmed neuronal morphology of the differentiated cells. Furthermore, real-time PCR and immunoflourescence approved the expression of neuron specific markers. P19-GFP+ cells were able to survive, migrate and integrated into host tissues when transplanted to developing chick embryo CNS. The obtained live GFP-expressing cells can be used as an abundant source of developmentally pluripotent material for transplantation studies, investigating the cellular and molecular aspects of early differentiation.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: Antiparkinson drugs, GFP, Neuronal differentiation ,Selegiline, Stem cells, Synaptophysin
Subjects: WL Nervous system
Divisions: Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Depositing User: zahra bagheri .
Date Deposited: 21 Jun 2017 06:42
Last Modified: 02 Jun 2018 09:36
URI: http://eprints.skums.ac.ir/id/eprint/176

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