High-throughput bioaccumulation, biotransformation, and production of silver and selenium nanoparticles using genetically engineered Pichia pastoris

Elahian, F. and Reiisi, S. and Shahidi, A. and Mirzaei, S.A. (2017) High-throughput bioaccumulation, biotransformation, and production of silver and selenium nanoparticles using genetically engineered Pichia pastoris. Nanomedicine: Nanotechnology, Biology, and Medicine, 13 (3). pp. 853-861.

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Abstract

A genetically modified Pichia pastoris strain overexpressing a metal-resistant variant of cytochrome b5 reductase enzyme was developed for silver and selenium biosorption and for nanoparticle production. The maximum recombinant enzyme expression level was approximately 31 IU/ml in the intercellular fluid after 24 h of incubation, and the capacity of the recombinant biomass for the biosorption of silver and selenium in aqueous batch models were measured as 163.90 and 63.71 mg/g, respectively. The ions were reduced in the presence of enzyme, leading to the formation of stable 70–180 nm metal nanoparticles. Various instrumental analyses confirmed the well-dispersed and crystalline nature of the spherical nanometals. The purified silver and selenium nanoparticles exhibited at least 10-fold less cytotoxicity toward HDF, EPG85–257, and T47D cells than silver nitrate and selenium dioxide. These results revealed that the engineered Pichia strain is an eco-friendly, rapid, high-throughput, and versatile reduction system for nanometal production.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: Biosorption ,Cytochrome b5 reductase, Heavy metals, Nanoparticle, Recombinant yeast
Subjects: W General medicine- Health professions > Medical Informatics , Information Systems W 26/55.I4
QU Biochemistry
Divisions: Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Depositing User: zahra bagheri .
Date Deposited: 21 Jun 2017 05:50
Last Modified: 28 Jun 2017 05:26
URI: http://eprints.skums.ac.ir/id/eprint/171

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