Molecular characterization of nosocomial Clostridium difficile infection in pediatric ward in Iran.

Khoshdel, Abolfazl. and Habibian, Roya. and Parvin, Neda. and Doosti, Abbas. and Famouri, Fatemeh. and Eshraghi, Ali. and Hafizi, Masoud. (2015) Molecular characterization of nosocomial Clostridium difficile infection in pediatric ward in Iran. SpringerPlus, 4. p. 627. ISSN 2193-1801

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Abstract

Clostridium difficile is recognized as a major cause of nosocomial acquired antibiotic-associated diarrhea and pseudomembranous colitis. It is a significant financial burden on modern healthcare resources. This study aimed to assess the molecular characterization of C. difficile strains isolated from children under 5 years old suffered from nosocomial diarrhea. One hundred diarrheic and 130 non-diarrheic fecal samples were collected from pediatrics less than 5 years old. Samples were cultured and C. difficile isolates were subjected to the PCR technique to study the distribution of ribotypes of C. difficile using P3 and P5 primers. Fifty-two out of 100 samples (52 %) were positive for C. difficile. The prevalence of bacterium in healthy children was 4.61 %. Total prevalence of C. difficile in diarrheic girls and boys were 48.9 and 54.7 %, respectively. Thirteen to twenty-four month age children had the highest prevalence of C. difficile. The most commonly detected ribotypes in the C. difficile isolates of Iranian pediatrics were RT027 (11.52 %), R1 (9.61 %) and R13 (7.68 %). The ribotypes of all of the six bacterial isolates of healthy children was not diagnosed. According to the presence of C. difficile and R27 ribotype, a continued genotype surveillance of this bacterium is necessary to monitor changes in the prevalence of certain strains and to identify the emergence of new strains that could affect future vaccine strategies.

Item Type: Article
Uncontrolled Keywords: Clostridium difficile; Diarrhea; Iran; Pediatrics; Ribotyping
Subjects: WC Communicable Diseases
WS Pediatrics
Divisions: Faculty of Medicine > Department of Clinical Sciences > Department of Infectious Diseases
Faculty of Medicine > Department of Clinical Sciences > Department of Internal Medicine
Faculty of Medicine > Department of Clinical Sciences > Department of Pediatrics
Faculty of Nursing and Midwifery
Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Reserach Vice-Chancellar Department > Clinical Biochemistry Research Center
Depositing User: Users 1 not found.
Date Deposited: 25 Jul 2017 06:58
Last Modified: 15 Apr 2018 05:01
URI: http://eprints.skums.ac.ir/id/eprint/1709

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