2,3,7,8-tetrachlorodibenzo-p-dioxin decrease expression of aryl hydrocarbon receptor in peripheral lymphocyte of β-thalassemia major patients.

Ghatreh samani, Mahdi. and Soleimani, Masoud. and Moayedi Esfahani, Behjat Al‑Sadat. and Ganjalikhani Hakemi, Mazdak. and shirzad, Hedayatollah. and Eskandari, Nahid. and Adib, Minoo. (2015) 2,3,7,8-tetrachlorodibenzo-p-dioxin decrease expression of aryl hydrocarbon receptor in peripheral lymphocyte of β-thalassemia major patients. Advanced biomedical research, 4. p. 218. ISSN 2277-9175

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Abstract

BACKGROUND β-thalassemia major is a hereditary disease with inefficient erythropoiesis. Level of inflammatory cytokine is elevated in these patients. In this study, we investigate the effect of aryl hydrocarbon receptor (AhR) ligand, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), on the expression of inflammatory mediators in β-thalassemia major patient's lymphocytes. MATERIALS AND METHODS Peripheral blood mononuclear cells of patients and healthy participants was isolated and cultured in favor of lymphocytes increment. Based on the treatment, we divided the cell into four groups. The orders of group's treatments were no treatment, tumor necrosis factor-α (TNF-α) treatment, TNF-α and TCDD treatment, TCDD treatment in Group 1-4, respectively. After cell culture, we extracted the cells RNA and converted them to cDNA. Real-time polymerase chain reaction was performed to assessment relative expression of caspase-1, NLRP3, and AhR. We compared all patient groups with equal healthy (control) groups. RESULTS Results showed that expression of caspase-1 in patients (Groups 1 and 2) was significantly lower than healthy individuals (P < 0.05). Although, no significant difference was found (Groups 1, 2, and control) in AhR gene expression (P > 0.05). Expression of AhR in other groups of patients (3 and 4) was significantly lower than control groups (P < 0.05). Expression of caspase-1 in Group 4 was significantly larger than the control group (P < 0.001). CONCLUSIONS We show here that chronic inflammation decrease caspase-1 expression and exposure of human lymphocytes to TCDD promote caspase-1 expression. Furthermore, activation of AhR with TCDD decreases AhR expression in lymphocytes of β-thalassemia major disease.

Item Type: Article
Uncontrolled Keywords: 2,3,7,8-tetrachlorodibenzo-p-dioxin; aryl hydrocarbon receptor; caspase-1; β-thalassemia major
Subjects: WH Hemic and Lymphatic System
QU Biochemistry
Divisions: Faculty of Medicine > Basic Sciences Academic Groups > Department of Immunology
Depositing User: Users 1 not found.
Date Deposited: 24 Jul 2017 10:27
Last Modified: 28 May 2018 05:42
URI: http://eprints.skums.ac.ir/id/eprint/1698

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