Aloe vera toxic effects: expression of inducible nitric oxide synthase (iNOS) in testis of Wistar rat.

Asgharzadeh, Samira. and Rafieian-Kopaei, Mahmoud. and Mirzaeian, Amin. and Reiisi, Somayeh. and Salimzadeh, Loghman. (2015) Aloe vera toxic effects: expression of inducible nitric oxide synthase (iNOS) in testis of Wistar rat. Iranian journal of basic medical sciences, 18 (10). pp. 967-73. ISSN 2008-3866

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Abstract

OBJECTIVES Nitric oxide (NO), a product of inducible nitric oxide synthase (iNOS), contributes in germ cell apoptosis. This study was aimed to evaluate the effects of Aloe vera gel (AVG) on male Wistar rat reproductive organ, serum NO level, and expression of iNOS gene in leydig cells. MATERIALS AND METHODS Adult male Wistar rats (n=36) were used for experiments in three groups. The experimental groups were orally administered with the AVG extract solution once-daily as follow: 150 mg.kg(-1); group A, 300 mg.kg(-1); group B, and only normal saline; group C (control group). They were mated with untreated females and the reproductive and chemical parameters were assessed for each group, including semen quality, serum testosterone, sperm fertility, gonad and body weight, serum NO concentration (by the Griess method), and iNOS gene expression (using RT-PCR). RESULTS The testes weight, serum testosterone, as well as sperm count and fertility of the AVG treated groups were significantly reduced when compared to the control (P<0.001). Concentration of serum NO was significantly increased (37.1±4.63 µM) in the administrated group with higher AVG concentration, compared to the control group (P<0.001; 10.19±0.87 µM); however, iNOS mRNA expression was increased in the treated animals (P<0.001). CONCLUSION iNOS may play a functional role in spermatogenesis via apoptosis, reducing sperm count, but further studies are needed to illustrate the mechanisms by which AVG exerts its negative effects on spermatogenesis and sperm quality.

Item Type: Article
Subjects: WJ Urogenital System
QU Biochemistry
QV pharmacology > QV 752 Pharmacognosy
Divisions: Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Reserach Vice-Chancellar Department > Medical Plants Research Center
Depositing User: zahra bagheri .
Date Deposited: 23 Jul 2017 09:05
Last Modified: 20 May 2018 10:19
URI: http://eprints.skums.ac.ir/id/eprint/1687

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