Mucosal interleukin-21 mRNA expression level is high in patients with Helicobacter pylori and is associated with the severity of gastritis

Bagheri, Nader. and Azadegan-Dehkordi, Fatemeh. and Shirzad, Mahsa. and Zamanzad, Behnam. and Rahimian, Ghorbanali. and Taghikhani, Afshin. and Rafieian-Kopaei, Mahmoud. and shirzad, Hedayatollah. (2015) Mucosal interleukin-21 mRNA expression level is high in patients with Helicobacter pylori and is associated with the severity of gastritis. CENTRAL EUROPEAN JOURNAL OF IMMUNOLOGY, 40 (1). pp. 61-67.

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Abstract

Helicobacter pylori (H. pylori) infection is associated with gastritis and marked infiltration of the gastric mucosa by several cytokines secreting inflammatory cells. Different clinical forms of the infection may reflect distinctive patterns of cytokine expression. Interleukin (IL)-17, IL-21, IL-22, and IL-23 have been reported to be involved in H. pylori-induced gastric mucosal inflammation, but the details and relationship to different patterns of inflammation and virulence factors remain unclear. The present study was launched to analyse IL-6 expression in H. pylori-infected and uninfected gastric patients and to investigate its correlation with chronic gastritis among H. pylori-infected patients. Total RNA was extracted from the gastric antrum biopsies of 48 H. pylori-infected patients and 38 H. pylori uninfected patients. Mucosal IL-21 mRNA expression level in H. pylori-infected and uninfected gastric biopsy was determined by real-time PCR. The presence of vacA (vacuolating cytotoxin A) and cagA (cytotoxin associated gene A) virulence factors were evaluated using PCR. Interleukin-21 mRNA expression was significantly high in biopsies of H. pylori-infected patients compared to H. pylori uninfected patients, and the mucosal IL-21 mRNA level was positively correlated with the grade of chronic inflammation. There was no association between virulence factors and IL-21 mRNA expression. We believe that IL-21 might be involved in the pathogenesis of H. pylori and might be an index of the severity of chronic gastritis.

Item Type: Article
Uncontrolled Keywords: Helicobacter pylori; gastritis; IL-21; virulence factors
Subjects: WI Digestive System
QU Biochemistry
Divisions: Faculty of Medicine > Department of Clinical Sciences > Department of Internal Medicine
Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Reserach Vice-Chancellar Department > Medical Plants Research Center
Depositing User: Users 1 not found.
Date Deposited: 23 Jul 2017 05:35
Last Modified: 30 Jan 2018 08:48
URI: http://eprints.skums.ac.ir/id/eprint/1667

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